EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
ジャーナル・号・ページ	Mol Cell, Vol. 75, Issue 6, Page 1256-11269.e7, Year 2019
掲載日	2019年9月19日
著者	Jingdong Cheng / Jochen Baßler / Paulina Fischer / Benjamin Lau / Nikola Kellner / Ruth Kunze / Sabine Griesel / Martina Kallas / Otto Berninghausen / Daniela Strauss / Roland Beckmann / Ed Hurt /
PubMed 要旨	Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome ...Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.
リンク	Mol Cell / PubMed:31378463
手法	EM (単粒子)
解像度	3.5 - 7.1 Å
構造データ	10051 6rxt EMDB-10051, PDB-6rxt: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state A 手法: EM (単粒子) / 解像度: 7.0 Å 10052 6rxu EMDB-10052, PDB-6rxu: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B1 手法: EM (単粒子) / 解像度: 3.5 Å 10053 6rxv EMDB-10053, PDB-6rxv: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B2 手法: EM (単粒子) / 解像度: 4.0 Å 10054 6rxx EMDB-10054: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state C PDB-6rxx: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state C, Poly-Ala 手法: EM (単粒子) / 解像度: 7.1 Å 10055 6rxy EMDB-10055, PDB-6rxy: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state a 手法: EM (単粒子) / 解像度: 4.7 Å 10056 6rxz EMDB-10056, PDB-6rxz: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state b 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	chaetomium thermophilum (菌類) chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (菌類)
キーワード	RIBOSOME (リボソーム) / ribosome biogenesis (リボソーム生合成) / rRNA (リボソームRNA)