EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM.
ジャーナル・号・ページ	Science, Vol. 364, Issue 6438, Page 355-362, Year 2019
掲載日	2019年4月26日
著者	Yan Zhao / Shanshuang Chen / Adam C Swensen / Wei-Jun Qian / Eric Gouaux /
PubMed 要旨	Glutamate-gated AMPA receptors mediate the fast component of excitatory signal transduction at chemical synapses throughout all regions of the mammalian brain. AMPA receptors are tetrameric ...Glutamate-gated AMPA receptors mediate the fast component of excitatory signal transduction at chemical synapses throughout all regions of the mammalian brain. AMPA receptors are tetrameric assemblies composed of four subunits, GluA1-GluA4. Despite decades of study, the subunit composition, subunit arrangement, and molecular structure of native AMPA receptors remain unknown. Here we elucidate the structures of 10 distinct native AMPA receptor complexes by single-particle cryo-electron microscopy (cryo-EM). We find that receptor subunits are arranged nonstochastically, with the GluA2 subunit preferentially occupying the B and D positions of the tetramer and with triheteromeric assemblies comprising a major population of native AMPA receptors. Cryo-EM maps define the structure for S2-M4 linkers between the ligand-binding and transmembrane domains, suggesting how neurotransmitter binding is coupled to ion channel gating.
リンク	Science / PubMed:30975770 / PubMed Central
手法	EM (単粒子)
解像度	4.5 - 32.0 Å
構造データ	EMDB-0426: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 12.8 Å EMDB-0427: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-0428: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 32.0 Å EMDB-0429: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 12.8 Å EMDB-0430: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 8.7 Å EMDB-0431: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-0432: Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-20085: structure of a complex 手法: EM (単粒子) / 解像度: 4.5 Å 9387 6njl EMDB-9387, PDB-6njl: Architecture and subunit arrangement of native AMPA receptors 手法: EM (単粒子) / 解像度: 6.7 Å 9388 6njm EMDB-9388, PDB-6njm: Architecture and subunit arrangement of native AMPA receptors 手法: EM (単粒子) / 解像度: 6.5 Å 9389 6njn EMDB-9389, PDB-6njn: Architecture and subunit arrangement of native AMPA receptors 手法: EM (単粒子) / 解像度: 6.5 Å
化合物	ChemComp-ZK1: {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid / ZK-200775 / アンタゴニスト, 薬剤YM / Fanapanel ChemComp-NAG*: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	rattus norvegicus (ドブネズミ) Rat (クマネズミ属) mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN/IMMUNE SYSTEM (生体膜) / AMPA receptor (AMPA型グルタミン酸受容体) / ligand gated ion channel (リガンド依存性イオンチャネル) / neurotransmitter (神経伝達物質) / synapse (シナプス) / MEMBRANE PROTEIN (膜タンパク質) / MEMBRANE PROTEIN-IMMUNE SYSTEM complex (生体膜)