EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural comparison of different antibodies interacting with parvovirus capsids.
ジャーナル・号・ページ	J Virol, Vol. 83, Issue 11, Page 5556-5566, Year 2009
掲載日	2009年3月25日
著者	Susan Hafenstein / Valorie D Bowman / Tao Sun / Christian D S Nelson / Laura M Palermo / Paul R Chipman / Anthony J Battisti / Colin R Parrish / Michael G Rossmann /
PubMed 要旨	The structures of canine parvovirus (CPV) and feline parvovirus (FPV) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron ...The structures of canine parvovirus (CPV) and feline parvovirus (FPV) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron microscopy (cryoEM) reconstruction to resolutions varying from 8.5 to 18 A. The crystal structure of one of the Fab molecules and the sequence of the variable domain for each of the Fab molecules have been determined. The structures of Fab fragments not determined crystallographically were predicted by homology modeling according to the amino acid sequence. Fitting of the Fab and virus structures into the cryoEM densities identified the footprints of each antibody on the viral surface. As anticipated from earlier analyses, the Fab binding sites are directed to two epitopes, A and B. The A site is on an exposed part of the surface near an icosahedral threefold axis, whereas the B site is about equidistant from the surrounding five-, three-, and twofold axes. One antibody directed to the A site binds CPV but not FPV. Two of the antibodies directed to the B site neutralize the virus as Fab fragments. The differences in antibody properties have been linked to the amino acids within the antibody footprints, the position of the binding site relative to the icosahedral symmetry elements, and the orientation of the Fab structure relative to the surface of the virus. Most of the exposed surface area was antigenic, although each of the antibodies had a common area of overlap that coincided with the positions of the previously mapped escape mutations.
リンク	J Virol / PubMed:19321620 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2 - 18.0 Å
構造データ	5105 3iy0 EMDB-5105: Canine parvovirus in complex with FAb from neutralizing antibody MAb 14 PDB-3iy0: Variable domains of the x-ray structure of Fab 14 fitted into the cryoEM reconstruction of the virus-Fab 14 complex 手法: EM (単粒子) / 解像度: 12.5 Å 5106 3iy1 EMDB-5106: Fab from MAb B interacting with feline panleukopenia virus PDB-3iy1: Variable domains of the WAM of Fab B fitted into the cryoEM reconstruction of the virus-Fab B complex 手法: EM (単粒子) / 解像度: 18.0 Å 5107 3iy2 EMDB-5107: Feline panleukopenia virus in complex with FAb from neutralizing antibody MAb 6 PDB-3iy2: Variable domains of the computer generated model (WAM) of Fab 6 fitted into the cryoEM reconstruction of the virus-Fab 6 complex 手法: EM (単粒子) / 解像度: 18.0 Å 5108 3iy3 EMDB-5108: Feline panleukopenia virus in complex with FAb from neutralizing antibody MAb 8 PDB-3iy3: Variable domains of the computer generated model (WAM) of Fab 8 fitted into the cryoEM reconstruction of the virus-Fab 8 complex 手法: EM (単粒子) / 解像度: 11.1 Å 5109 3iy4 EMDB-5109: Feline panleukopenia virus in complex with FAb from neutralizing antibody MAb 15 PDB-3iy4: Variable domains of the computer generated model (WAM) of Fab 15 fitted into the cryoEM reconstruction of the virus-Fab 15 complex 手法: EM (単粒子) / 解像度: 11.7 Å 5110 3iy5 EMDB-5110: Feline panleukopenia virus in complex with FAb from neutralizing antibody MAb 16 PDB-3iy5: Variable domains of the mouse Fab (1AIF) fitted into the cryoEM reconstruction of the virus-Fab 16 complex 手法: EM (単粒子) / 解像度: 18.0 Å 5111 3iy6 EMDB-5111: Feline panleukopenia virus in complex with FAb from neutralizing antibody MAb E PDB-3iy6: Variable domains of the computer generated model (WAM) of Fab E fitted into the cryoEM reconstruction of the virus-Fab E complex 手法: EM (単粒子) / 解像度: 12.0 Å 5112 3iy7 EMDB-5112: Feline panleukopenia virus in complex with FAb from neutralizing antibody MAb F PDB-3iy7: Variable domains of the computer generated model (WAM) of Fab F fitted into the cryoEM reconstruction of the virus-Fab F complex 手法: EM (単粒子) / 解像度: 14.0 Å PDB-3gk8: X-ray crystal structure of the Fab from MAb 14, mouse antibody against Canine Parvovirus 手法: X-RAY DIFFRACTION / 解像度: 2.0 Å
化合物	ChemComp-HOH: WATER / 水
由来	mus musculus (ハツカネズミ) rattus norvegicus (ドブネズミ)
キーワード	IMMUNE SYSTEM (免疫系) / antibody fragment from neutralizing monoclonal antibody against canine parvovirus / cryoEM (低温電子顕微鏡法) / neutralizing antibody (中和抗体) / parvovirus (パルボウイルス) / canine / feline / fab footprint