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Structure paper

タイトル	Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 6904, Year 2022
掲載日	2022年11月12日
著者	Jonathan Mount / Grigory Maksaev / Brock T Summers / James A J Fitzpatrick / Peng Yuan /
PubMed 要旨	Mechanosensitive channels of small conductance, found in many living organisms, open under elevated membrane tension and thus play crucial roles in biological response to mechanical stress. Amongst ...Mechanosensitive channels of small conductance, found in many living organisms, open under elevated membrane tension and thus play crucial roles in biological response to mechanical stress. Amongst these channels, MscK is unique in that its activation also requires external potassium ions. To better understand this dual gating mechanism by force and ligand, we elucidate distinct structures of MscK along the gating cycle using cryo-electron microscopy. The heptameric channel comprises three layers: a cytoplasmic domain, a periplasmic gating ring, and a markedly curved transmembrane domain that flattens and expands upon channel opening, which is accompanied by dilation of the periplasmic ring. Furthermore, our results support a potentially unifying mechanotransduction mechanism in ion channels depicted as flattening and expansion of the transmembrane domain.
リンク	Nat Commun / PubMed:36371466 / PubMed Central
手法	EM (単粒子)
解像度	3.41 - 4.97 Å
構造データ	26823 7uw5 EMDB-26823, PDB-7uw5: EcMscK G924S mutant in a closed conformation 手法: EM (単粒子) / 解像度: 3.84 Å 26845 7ux1 EMDB-26845, PDB-7ux1: EcMscK in an Open Conformation 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-26851: WT EcMscK in a closed conformation 手法: EM (単粒子) / 解像度: 4.48 Å EMDB-26854: EcMscK in an intermediate conformation 手法: EM (単粒子) / 解像度: 4.97 Å EMDB-26872: Locally refined core of EcMscK in a closed conformation 手法: EM (単粒子) / 解像度: 3.42 Å EMDB-26875: Locally refined core of EcMscK G924S in a closed conformation 手法: EM (単粒子) / 解像度: 3.54 Å EMDB-26876: Locally refined core of EcMscK G924S in an intermediate conformation 手法: EM (単粒子) / 解像度: 3.85 Å EMDB-26877: Locally refined core of EcMscK in an open conformation 手法: EM (単粒子) / 解像度: 3.41 Å
由来	escherichia coli k-12 (大腸菌)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / MEMBRANE PROTEIN (膜タンパク質) / MECHANOSENSATION / ION CHANNEL (イオンチャネル)