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Structure paper

タイトル	Structures of mammalian RNA polymerase II pre-initiation complexes.
ジャーナル・号・ページ	Nature, Vol. 594, Issue 7861, Page 124-128, Year 2021
掲載日	2021年4月26日
著者	Shintaro Aibara / Sandra Schilbach / Patrick Cramer /
PubMed 要旨	The initiation of transcription is a focal point for the regulation of gene activity during mammalian cell differentiation and development. To initiate transcription, RNA polymerase II (Pol II) ...The initiation of transcription is a focal point for the regulation of gene activity during mammalian cell differentiation and development. To initiate transcription, RNA polymerase II (Pol II) assembles with general transcription factors into a pre-initiation complex (PIC) that opens promoter DNA. Previous work provided the molecular architecture of the yeast and human PIC and a topological model for DNA opening by the general transcription factor TFIIH. Here we report the high-resolution cryo-electron microscopy structure of PIC comprising human general factors and Sus scrofa domesticus Pol II, which is 99.9% identical to human Pol II. We determine the structures of PIC with closed and opened promoter DNA at 2.5-2.8 Å resolution, and resolve the structure of TFIIH at 2.9-4.0 Å resolution. We capture the TFIIH translocase XPB in the pre- and post-translocation states, and show that XPB induces and propagates a DNA twist to initiate the opening of DNA approximately 30 base pairs downstream of the TATA box. We also provide evidence that DNA opening occurs in two steps and leads to the detachment of TFIIH from the core PIC, which may stop DNA twisting and enable RNA chain initiation.
リンク	Nature / PubMed:33902107
手法	EM (単粒子)
解像度	2.4 - 9.1 Å
構造データ	12610 7nvr EMDB-12610, PDB-7nvr: Human Mediator with RNA Polymerase II Pre-initiation complex 手法: EM (単粒子) / 解像度: 4.5 Å 12611 7nvs EMDB-12611, PDB-7nvs: RNA polymerase II core pre-initiation complex with closed promoter DNA in proximal position 手法: EM (単粒子) / 解像度: 2.8 Å 12612 7nvt EMDB-12612, PDB-7nvt: RNA polymerase II core pre-initiation complex with closed promoter DNA in distal position 手法: EM (単粒子) / 解像度: 2.9 Å 12613 7nvu EMDB-12613, PDB-7nvu: RNA polymerase II core pre-initiation complex with open promoter DNA 手法: EM (単粒子) / 解像度: 2.5 Å 12614 7nvv EMDB-12614, PDB-7nvv: XPB-containing part of TFIIH in a post-translocated state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.9 Å 12615 7nvw EMDB-12615, PDB-7nvw: TFIIH in a pre-translocated state (without ADP-BeF3) 手法: EM (単粒子) / 解像度: 4.3 Å 12616 7nvx EMDB-12616, PDB-7nvx: TFIIH in a post-translocated state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 3.9 Å 12617 7nvy EMDB-12617, PDB-7nvy: RNA polymerase II pre-initiation complex with closed promoter DNA in proximal position 手法: EM (単粒子) / 解像度: 7.3 Å 12618 7nvz EMDB-12618, PDB-7nvz: RNA polymerase II pre-initiation complex with closed promoter DNA in distal position 手法: EM (単粒子) / 解像度: 7.2 Å 12619 7nw0 EMDB-12619, PDB-7nw0: RNA polymerase II pre-initiation complex with open promoter DNA 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-12620: Focused refinement of the core RNA polymerase II pre-initiation complex in a consensus CC state (without ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-12621: Focused refinement of the core RNA polymerase II pre-initiation complex in a consensus OC state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-12622: Focused refinement of the upstream complex for the RNA polymerase II pre-initiation complex in a consensus CC state (without ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-12623: Focused refinement of the upstream complex for the RNA polymerase II pre-initiation complex in a consensus OC state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-12624: Focused refinement of XPB containing part of TFIIH in pre-translocated state (without ADP-BeF3) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-12625: Focused refinement of XPD containing part of TFIIH in pre-translocated state (without ADP-BeF3) 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-12626: Focused refinement of XPD containing part of TFIIH in post-translocated state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-12627: Focused refinement of the RNA polymerase II core pre-initiation complex in a consensus IC-like state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-12628: Focused refinement of the upstream complex for the RNA polymerase II pre-initiation complex in a consensus IC-like state (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-12629: Focused refinement of the core RNA polymerase II pre-initiation complex with intermediary promoter DNA (with ADP-BeF3) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-12630: RNA polymerase II pre-initiation complex with intermediary promoter DNA 手法: EM (単粒子) / 解像度: 9.1 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF*: BERYLLIUM TRIFLUORIDE ION
由来	homo sapiens (ヒト) sus scrofa (ブタ) human mastadenovirus c (ウイルス) trichoplusia ni (イラクサキンウワバ) Pig (ブタ) unidentified adenovirus (ウイルス)
キーワード	TRANSCRIPTION (転写 (生物学)) / Mediator complex / RNA polymerase II (RNAポリメラーゼII) / TFIIH (TFIIH) / Pre-initiation complex / Initiation