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Structure paper

タイトル	Cryo-EM structure of native human uromodulin, a zona pellucida module polymer.
ジャーナル・号・ページ	EMBO J, Vol. 39, Issue 24, Page e106807, Year 2020
掲載日	2020年12月15日
著者	Alena Stsiapanava / Chenrui Xu / Martina Brunati / Sara Zamora-Caballero / Céline Schaeffer / Marcel Bokhove / Ling Han / Hans Hebert / Marta Carroni / Shigeki Yasumasu / Luca Rampoldi / Bin Wu / Luca Jovine /
PubMed 要旨	Assembly of extracellular filaments and matrices mediating fundamental biological processes such as morphogenesis, hearing, fertilization, and antibacterial defense is driven by a ubiquitous ...Assembly of extracellular filaments and matrices mediating fundamental biological processes such as morphogenesis, hearing, fertilization, and antibacterial defense is driven by a ubiquitous polymerization module known as zona pellucida (ZP) "domain". Despite the conservation of this element from hydra to humans, no detailed information is available on the filamentous conformation of any ZP module protein. Here, we report a cryo-electron microscopy study of uromodulin (UMOD)/Tamm-Horsfall protein, the most abundant protein in human urine and an archetypal ZP module-containing molecule, in its mature homopolymeric state. UMOD forms a one-start helix with an unprecedented 180-degree twist between subunits enfolded by interdomain linkers that have completely reorganized as a result of propeptide dissociation. Lateral interaction between filaments in the urine generates sheets exposing a checkerboard of binding sites to capture uropathogenic bacteria, and UMOD-based models of heteromeric vertebrate egg coat filaments identify a common sperm-binding region at the interface between subunits.
リンク	EMBO J / PubMed:33196145 / PubMed Central
手法	EM (らせん対称)
解像度	3.35 - 3.96 Å
構造データ	10553 6tqk EMDB-10553, PDB-6tqk: Cryo-EM of native human uromodulin (UMOD)/Tamm-Horsfall protein (THP) filament. 手法: EM (らせん対称) / 解像度: 3.35 Å 10554 6tql EMDB-10554, PDB-6tql: Cryo-EM of elastase-treated human uromodulin (UMOD)/Tamm-Horsfall protein (THP) filament 手法: EM (らせん対称) / 解像度: 3.96 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	STRUCTURAL PROTEIN (タンパク質) / ZP MODULE / ZP DOMAIN / ZP-N DOMAIN / ZP-C DOMAIN / INTERDOMAIN LINKER / EGF DOMAIN (EGF様ドメイン) / EXTRACELLULAR MATRIX (細胞外マトリックス) / GLYCOPROTEIN (糖タンパク質) / N-GLYCAN (N-結合型グリコシル化) / PROTEIN FILAMENT / PROTEIN POLYMERIZATION