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Structure paper

タイトル	Structure of nucleosome-bound human BAF complex.
ジャーナル・号・ページ	Science, Vol. 367, Issue 6480, Page 875-881, Year 2020
掲載日	2020年2月21日
著者	Shuang He / Zihan Wu / Yuan Tian / Zishuo Yu / Jiali Yu / Xinxin Wang / Jie Li / Bijun Liu / Yanhui Xu /
PubMed 要旨	Mammalian SWI/SNF family chromatin remodelers, BRG1/BRM-associated factor (BAF) and polybromo-associated BAF (PBAF), regulate chromatin structure and transcription, and their mutations are linked to ...Mammalian SWI/SNF family chromatin remodelers, BRG1/BRM-associated factor (BAF) and polybromo-associated BAF (PBAF), regulate chromatin structure and transcription, and their mutations are linked to cancers. The 3.7-angstrom-resolution cryo-electron microscopy structure of human BAF bound to the nucleosome reveals that the nucleosome is sandwiched by the base and the adenosine triphosphatase (ATPase) modules, which are bridged by the actin-related protein (ARP) module. The ATPase motor is positioned proximal to nucleosomal DNA and, upon ATP hydrolysis, engages with and pumps DNA along the nucleosome. The C-terminal α helix of SMARCB1, enriched in positively charged residues frequently mutated in cancers, mediates interactions with an acidic patch of the nucleosome. AT-rich interactive domain-containing protein 1A (ARID1A) and the SWI/SNF complex subunit SMARCC serve as a structural core and scaffold in the base module organization, respectively. Our study provides structural insights into subunit organization and nucleosome recognition of human BAF complex.
リンク	Science / PubMed:32001526
手法	EM (単粒子)
解像度	3.0 - 10.3 Å
構造データ	0968 6lth EMDB-0968, PDB-6lth: Structure of human BAF Base module 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-0969: Structure of human BAF ARP module 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-0970: Structure of nucleosome in BAF-NCP complex 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-0971: Structure of nucleosome-bound human full length BAF complex 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-0972: Structure of nucleosome with DNA detachment in BAF-NCP complex 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-0973: Structure of nucleosome-bound human BAF complex in ADP-bound state 手法: EM (単粒子) / 解像度: 10.3 Å 0974 6ltj EMDB-0974, PDB-6ltj: Structure of nucleosome-bound human BAF complex 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト) Xenopus calcaratus (カエル) xenopus laevis (アフリカツメガエル)
キーワード	GENE REGULATION (遺伝子発現の調節) / Chromatin remodeler / Complex