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キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

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Structure paper

タイトル	Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 116, Issue 42, Page 21037-21046, Year 2019
掲載日	2019年10月15日
著者	Alexis Huet / Robert L Duda / Pascale Boulanger / James F Conway /
PubMed 要旨	The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a ...The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a regulated process that involves several intermediates, including a thick-walled round precursor prohead that expands as the viral DNA is packaged to yield a thin-walled and angular mature capsid. We investigated capsid maturation by comparing cryoelectron microscopy (cryo-EM) structures of the prohead, the empty expanded capsid both with and without decoration protein, and the virion capsid at a resolution of 3.8 Å for the latter. We detail the molecular structure of the mcp, its complex pattern of interactions, and their evolution during maturation. The bacteriophage T5 mcp is a variant of the canonical HK97-fold with a high level of plasticity that allows for the precise assembly of a giant macromolecule and the adaptability needed to interact with other proteins and the packaged DNA.
リンク	Proc Natl Acad Sci U S A / PubMed:31578255 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 7.2 Å
構造データ	20099 6okb EMDB-20099, PDB-6okb: Prohead 2 of the phage T5 手法: EM (単粒子) / 解像度: 6.7 Å 20122 6oma EMDB-20122, PDB-6oma: non-decorated head of the phage T5 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-20123: decorated head of the phage T5 手法: EM (単粒子) / 解像度: 6.8 Å 20125 6omc EMDB-20125, PDB-6omc: capsid of T5 virion 手法: EM (単粒子) / 解像度: 3.8 Å
由来	escherichia phage t5 (ファージ)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / procapsid (カプシド) / HK97-fold / dsDNA-phage / icosahedral (二十面体) / VIRUS (ウイルス) / capsid (カプシド)