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Structure paper

タイトル	Structure of the Cdc48 segregase in the act of unfolding an authentic substrate.
ジャーナル・号・ページ	Science, Vol. 365, Issue 6452, Page 502-505, Year 2019
掲載日	2019年8月2日
著者	Ian Cooney / Han Han / Michael G Stewart / Richard H Carson / Daniel T Hansen / Janet H Iwasa / John C Price / Christopher P Hill / Peter S Shen /
PubMed 要旨	The cellular machine Cdc48 functions in multiple biological pathways by segregating its protein substrates from a variety of stable environments such as organelles or multi-subunit complexes. Despite ...The cellular machine Cdc48 functions in multiple biological pathways by segregating its protein substrates from a variety of stable environments such as organelles or multi-subunit complexes. Despite extensive studies, the mechanism of Cdc48 has remained obscure, and its reported structures are inconsistent with models of substrate translocation proposed for other AAA+ ATPases (adenosine triphosphatases). Here, we report a 3.7-angstrom-resolution structure of Cdc48 in complex with an adaptor protein and a native substrate. Cdc48 engages substrate by adopting a helical configuration of substrate-binding residues that extends through the central pore of both of the ATPase rings. These findings indicate a unified hand-over-hand mechanism of protein translocation by Cdc48 and other AAA+ ATPases.
リンク	Science / PubMed:31249134 / PubMed Central
手法	EM (単粒子)
解像度	3.7 - 4.5 Å
構造データ	20124 6omb EMDB-20124: Cdc48 Hexamer PDB-6omb: Cdc48 Hexamer (Subunits A to E) with substrate bound to the central pore 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-20136: Cdc48 Hexamer (Focused Classification for Subunit F, state1) 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-20137: Cdc48 Hexamer (Focused Classification for Subunit F, state2) 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-20138: Cdc48 Symmetric Hexamer 手法: EM (単粒子) / 解像度: 4.5 Å 20149 6opc EMDB-20149, PDB-6opc: Cdc48 Hexamer in a complex with substrate and Shp1(Ubx Domain) 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) saccharomyces cerevisiae (パン酵母)
キーワード	MOTOR PROTEIN (モータータンパク質) / Cdc48 / AAA+ ATPase / substrate translocation