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Structure paper

タイトル	Calicivirus VP2 forms a portal-like assembly following receptor engagement.
ジャーナル・号・ページ	Nature, Vol. 565, Issue 7739, Page 377-381, Year 2019
掲載日	2019年1月9日
著者	Michaela J Conley / Marion McElwee / Liyana Azmi / Mads Gabrielsen / Olwyn Byron / Ian G Goodfellow / David Bhella /
PubMed 要旨	To initiate infection, many viruses enter their host cells by triggering endocytosis following receptor engagement. However, the mechanisms by which non-enveloped viruses escape the endosome are ...To initiate infection, many viruses enter their host cells by triggering endocytosis following receptor engagement. However, the mechanisms by which non-enveloped viruses escape the endosome are poorly understood. Here we present near-atomic-resolution cryo-electron microscopy structures for feline calicivirus both undecorated and labelled with a soluble fragment of its cellular receptor, feline junctional adhesion molecule A. We show that VP2, a minor capsid protein encoded by all caliciviruses, forms a large portal-like assembly at a unique three-fold axis of symmetry, following receptor engagement. This assembly-which was not detected in undecorated virions-is formed of twelve copies of VP2, arranged with their hydrophobic N termini pointing away from the virion surface. Local rearrangement at the portal site leads to the opening of a pore in the capsid shell. We hypothesize that the portal-like assembly functions as a channel for the delivery of the calicivirus genome, through the endosomal membrane, into the cytoplasm of a host cell, thereby initiating infection. VP2 was previously known to be critical for the production of infectious virus; our findings provide insights into its structure and function that advance our understanding of the Caliciviridae.
リンク	Nature / PubMed:30626974
手法	EM (単粒子)
解像度	3.0 - 3.75 Å
構造データ	0054 6gsh EMDB-0054, PDB-6gsh: Feline Calicivirus Strain F9 手法: EM (単粒子) / 解像度: 3.0 Å 0056 6gsi EMDB-0056, PDB-6gsi: Feline Calicivirus Strain F9 bound to a soluble ectodomain fragment of feline junctional adhesion molecule A - leading to assembly of a portal structure at a unique three-fold axis. 手法: EM (単粒子) / 解像度: 3.75 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン
由来	feline calicivirus (ネコカリシウイルス) felis catus (イエネコ) feline calicivirus strain f9 (ネコカリシウイルス) FCV (ネコカリシウイルス)
キーワード	VIRUS (ウイルス) / Capsid (カプシド) / Calicivirus (カリシウイルス科) / Vesivirus / Vp1 / portal / Vp2 / junctional-adhesion molecule A