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Structure paper

タイトル	Architecture of the CBF3-centromere complex of the budding yeast kinetochore.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 25, Issue 12, Page 1103-1110, Year 2018
掲載日	2018年11月26日
著者	Kaige Yan / Ziguo Zhang / Jing Yang / Stephen H McLaughlin / David Barford /
PubMed 要旨	Kinetochores are multicomponent complexes responsible for coordinating the attachment of centromeric DNA to mitotic-spindle microtubules. The point centromeres of budding yeast are organized into ...Kinetochores are multicomponent complexes responsible for coordinating the attachment of centromeric DNA to mitotic-spindle microtubules. The point centromeres of budding yeast are organized into three centromeric determining elements (CDEs), and are associated with the centromere-specific nucleosome Cse4. Deposition of Cse4 at CEN loci is dependent on the CBF3 complex that engages CDEIII to direct Cse4 nucleosomes to CDEII. To understand how CBF3 recognizes CDEIII and positions Cse4, we determined a cryo-EM structure of a CBF3-CEN complex. CBF3 interacts with CEN DNA as a head-to-head dimer that includes the whole of CDEIII and immediate 3' regions. Specific CEN-binding of CBF3 is mediated by a Cep3 subunit of one of the CBF3 protomers that forms major groove interactions with the conserved and essential CCG and TGT motifs of CDEIII. We propose a model for a CBF3-Cse4-CEN complex with implications for understanding CBF3-directed deposition of the Cse4 nucleosome at CEN loci.
リンク	Nat Struct Mol Biol / PubMed:30478265 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 8.5 Å
構造データ	0095 6gyp EMDB-0095, PDB-6gyp: Cryo-EM structure of the CBF3-core-Ndc10-DBD complex of the budding yeast kinetochore 手法: EM (単粒子) / 解像度: 3.6 Å 0096 6gys EMDB-0096, PDB-6gys: Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore 手法: EM (単粒子) / 解像度: 4.4 Å 0097 6gyu EMDB-0097, PDB-6gyu: Cryo-EM structure of the CBF3-msk complex of the budding yeast kinetochore 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-11626: Structure of inner kinetochore CCAN-Cenp-A complex (Cenp-HIKTW) 手法: EM (単粒子) / 解像度: 8.5 Å
化合物	ChemComp-MET: METHIONINE / メチオニン / メチオニン ChemComp-PHE: PHENYLALANINE / フェニルアラニン / フェニルアラニン ChemComp-ASN: ASPARAGINE / アスパラギン / アスパラギン ChemComp-ARG: ARGININE / L-アルギニン-ω′-カチオン / アルギニン ChemComp-THR: THREONINE / トレオニン / トレオニン ChemComp-GLN: GLUTAMINE / グルタミン / グルタミン ChemComp-ZN: Unknown entry
由来	saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	CELL CYCLE (細胞周期) / Complex / DNA BINDING PROTEIN (DNA結合タンパク質)