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キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

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Structure paper

タイトル	Cryo-EM Structure of the Human Ribonuclease P Holoenzyme.
ジャーナル・号・ページ	Cell, Vol. 175, Issue 5, Page 1393-1404.e11, Year 2018
掲載日	2018年11月15日
著者	Jian Wu / Shuangshuang Niu / Ming Tan / Chenhui Huang / Mingyue Li / Yang Song / Qianmin Wang / Juan Chen / Shaohua Shi / Pengfei Lan / Ming Lei /
PubMed 要旨	Ribonuclease (RNase) P is a ubiquitous ribozyme that cleaves the 5' leader from precursor tRNAs. Here, we report cryo-electron microscopy structures of the human nuclear RNase P alone and in ...Ribonuclease (RNase) P is a ubiquitous ribozyme that cleaves the 5' leader from precursor tRNAs. Here, we report cryo-electron microscopy structures of the human nuclear RNase P alone and in complex with tRNA. Human RNase P is a large ribonucleoprotein complex that contains 10 protein components and one catalytic RNA. The protein components form an interlocked clamp that stabilizes the RNA in a conformation optimal for substrate binding. Human RNase P recognizes the tRNA using a double-anchor mechanism through both protein-RNA and RNA-RNA interactions. Structural comparison of the apo and tRNA-bound human RNase P reveals that binding of tRNA induces a local conformational change in the catalytic center, transforming the ribozyme into an active state. Our results also provide an evolutionary model depicting how auxiliary RNA elements in bacterial RNase P, essential for substrate binding, and catalysis, were replaced by the much more complex and multifunctional protein components in higher organisms.
リンク	Cell / PubMed:30454648
手法	EM (単粒子) / X線回折
解像度	2.6 - 3.92 Å
構造データ	9626 6ahr EMDB-9626, PDB-6ahr: Cryo-EM structure of human Ribonuclease P 手法: EM (単粒子) / 解像度: 3.92 Å 9627 6ahu EMDB-9627, PDB-6ahu: Cryo-EM structure of human Ribonuclease P with mature tRNA 手法: EM (単粒子) / 解像度: 3.66 Å PDB-6ahv: Crystal structure of human RPP40 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト)
キーワード	HYDROLASE/RNA / Ribonuclease P (リボヌクレアーゼP) / RNA-protein complex / HYDROLASE-RNA complex / HYDROLASE (加水分解酵素) / RNase P (リボヌクレアーゼP)