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Structure paper

タイトル	Structural Insights into Mdn1, an Essential AAA Protein Required for Ribosome Biogenesis.
ジャーナル・号・ページ	Cell, Vol. 175, Issue 3, Page 822-834.e18, Year 2018
掲載日	2018年10月18日
著者	Zhen Chen / Hiroshi Suzuki / Yuki Kobayashi / Ashley C Wang / Frank DiMaio / Shigehiro A Kawashima / Thomas Walz / Tarun M Kapoor /
PubMed 要旨	Mdn1 is an essential AAA (ATPase associated with various activities) protein that removes assembly factors from distinct precursors of the ribosomal 60S subunit. However, Mdn1's large size (∼5,000 ...Mdn1 is an essential AAA (ATPase associated with various activities) protein that removes assembly factors from distinct precursors of the ribosomal 60S subunit. However, Mdn1's large size (∼5,000 amino acid [aa]) and its limited homology to other well-studied proteins have restricted our understanding of its remodeling function. Here, we present structures for S. pombe Mdn1 in the presence of AMPPNP at up to ∼4 Å or ATP plus Rbin-1, a chemical inhibitor, at ∼8 Å resolution. These data reveal that Mdn1's MIDAS domain is tethered to its ring-shaped AAA domain through an ∼20 nm long structured linker and a flexible ∼500 aa Asp/Glu-rich motif. We find that the MIDAS domain, which also binds other ribosome-assembly factors, docks onto the AAA ring in a nucleotide state-specific manner. Together, our findings reveal how conformational changes in the AAA ring can be directly transmitted to the MIDAS domain and thereby drive the targeted release of assembly factors from ribosomal 60S-subunit precursors.
リンク	Cell / PubMed:30318141 / PubMed Central
手法	EM (単粒子)
解像度	4.0 - 7.7 Å
構造データ	9032 6or5 EMDB-9032, PDB-6or5: Full-length S. pombe Mdn1 in the presence of AMPPNP (ring region) 手法: EM (単粒子) / 解像度: 4.0 Å 9033 6or6 EMDB-9033, PDB-6or6: Full-length S. pombe Mdn1 in the presence of AMPPNP (tail region) 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-9034: C-terminally truncated S. pombe Mdn1 (1-3911 aa) in the presence of AMPPNP (ring region) 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-9035: C-terminally truncated S. pombe Mdn1 (1-3911 aa) in the presence of AMPPNP (tail region) 手法: EM (単粒子) / 解像度: 7.4 Å 9036 6orb EMDB-9036, PDB-6orb: Full-length S. pombe Mdn1 in the presence of ATP and Rbin-1 手法: EM (単粒子) / 解像度: 7.2 Å
化合物	ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	schizosaccharomyces pombe (分裂酵母) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (分裂酵母)
キーワード	MOTOR PROTEIN (モータータンパク質) / Ribosome biogenesis (リボソーム生合成) / AAA protein / Mechanochemical enzyme / Ribosome assembly factor