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キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

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Structure paper

タイトル	Structural insight into precursor tRNA processing by yeast ribonuclease P.
ジャーナル・号・ページ	Science, Vol. 362, Issue 6415, Year 2018
掲載日	2018年11月9日
著者	Pengfei Lan / Ming Tan / Yuebin Zhang / Shuangshuang Niu / Juan Chen / Shaohua Shi / Shuwan Qiu / Xuejuan Wang / Xiangda Peng / Gang Cai / Hong Cheng / Jian Wu / Guohui Li / Ming Lei /
PubMed 要旨	Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of ...Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of RNase P alone and in complex with pre-tRNA The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion S2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P.
リンク	Science / PubMed:30262633
手法	EM (単粒子)
解像度	3.48 Å
構造データ	9616 6agb EMDB-9616, PDB-6agb: Cryo-EM structure of yeast Ribonuclease P 手法: EM (単粒子) / 解像度: 3.48 Å 9622 6ah3 EMDB-9622, PDB-6ah3: Cryo-EM structure of yeast Ribonuclease P with pre-tRNA substrate 手法: EM (単粒子) / 解像度: 3.48 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) Baker's yeast (パン酵母) saccharomyces cerevisiae s288c (パン酵母)
キーワード	HYDROLASE/RNA / Ribonuclease P (リボヌクレアーゼP) / RNA-protein complex / HYDROLASE-RNA complex