+
検索条件

キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

-
Structure paper

タイトル	Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
ジャーナル・号・ページ	Science, Vol. 362, Issue 6413, Year 2018
掲載日	2018年10月26日
著者	David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban /
PubMed 要旨	Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
リンク	Science / PubMed:30213880
手法	EM (単粒子)
解像度	3.08 - 7.8 Å
構造データ	0229 6hiv EMDB-0229, PDB-6hiv: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete mitoribosome 手法: EM (単粒子) / 解像度: 7.8 Å 0230 6hiw EMDB-0230, PDB-6hiw: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete small mitoribosomal subunit in complex with mt-IF-3 手法: EM (単粒子) / 解像度: 3.37 Å 0231 6hix EMDB-0231, PDB-6hix: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit 手法: EM (単粒子) / 解像度: 3.39 Å 0232 6hiy EMDB-0232, PDB-6hiy: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the body of the small mitoribosomal subunit in complex with mt-IF-3 手法: EM (単粒子) / 解像度: 3.27 Å 0233 6hiz EMDB-0233, PDB-6hiz: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit 手法: EM (単粒子) / 解像度: 3.08 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SPD: SPERMIDINE / スペルミジン / スペルミジン ChemComp-UTP: URIDINE 5'-TRIPHOSPHATE / UTP / UTPYM / ウリジン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-SPM: SPERMINE / スペルミン / スペルミン ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM / ニコチンアミドアデニンジヌクレオチド ChemComp-SER: SERINE / セリン / セリン ChemComp-HOH*: WATER / 水
由来	trypanosoma brucei brucei (ブルーストリパノソーマ)
キーワード	RIBOSOME (リボソーム) / mitoribosome / translation (翻訳 (生物学)) / Trypanosoma (トリパノソーマ) / large ribosomal subunit / small ribosomal subunit / 12S rRNA / 9S rRNA / ribosomal protein / mitochondrial initiation factor IF-3