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-Structure paper
タイトル | Cryo-EM structure of a mitochondrial calcium uniporter. |
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ジャーナル・号・ページ | Science, Vol. 361, Issue 6401, Page 506-511, Year 2018 |
掲載日 | 2018年8月3日 |
著者 | Jiho Yoo / Mengyu Wu / Ying Yin / Mark A Herzik / Gabriel C Lander / Seok-Yong Lee / |
PubMed 要旨 | Calcium transport plays an important role in regulating mitochondrial physiology and pathophysiology. The mitochondrial calcium uniporter (MCU) is a calcium-selective ion channel that is the primary ...Calcium transport plays an important role in regulating mitochondrial physiology and pathophysiology. The mitochondrial calcium uniporter (MCU) is a calcium-selective ion channel that is the primary mediator for calcium uptake into the mitochondrial matrix. Here, we present the cryo-electron microscopy structure of the full-length MCU from to an overall resolution of ~3.7 angstroms. Our structure reveals a tetrameric architecture, with the soluble and transmembrane domains adopting different symmetric arrangements within the channel. The conserved W-D-Φ-Φ-E-P-V-T-Y sequence motif of MCU pore forms a selectivity filter comprising two acidic rings separated by one helical turn along the central axis of the channel pore. The structure combined with mutagenesis gives insight into the basis of calcium recognition. |
リンク | Science / PubMed:29954988 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.7 Å |
構造データ | |
化合物 | ChemComp-CA: |
由来 |
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キーワード | TRANSPORT PROTEIN (運搬体タンパク質) / mitochondrial calcium uniporter / calcium-selective ion channel / calcium uptake / uniporter |