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Structure paper

タイトル	Structure and assembly of the Ebola virus nucleocapsid.
ジャーナル・号・ページ	Nature, Vol. 551, Issue 7680, Page 394-397, Year 2017
掲載日	2017年11月16日
著者	William Wan / Larissa Kolesnikova / Mairi Clarke / Alexander Koehler / Takeshi Noda / Stephan Becker / John A G Briggs /
PubMed 要旨	Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles ...Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein-nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein, and cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended α-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment.
リンク	Nature / PubMed:29144446 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	6.6 - 9.1 Å
構造データ	3869 6ehl EMDB-3869: The structure of Ebola virus nucleoprotein (residues 1-450) in nucleocapsid-like assemblies PDB-6ehl: Model of the Ebola virus nucleoprotein in recombinant nucleocapsid-like assemblies 手法: EM (サブトモグラム平均) / 解像度: 6.6 Å EMDB-3870: Cryo-electron tomogram of Ebola virus nucleoprotein, residues 1-450 手法: EM (トモグラフィー) 3871 6ehm EMDB-3871: The structure of Ebola virus nucleocapsid-like assemblies from recombinant virus-like particles (nucleoprotein, VP24,VP35,VP40) PDB-6ehm: Model of the Ebola virus nucleocapsid subunit from recombinant virus-like particles 手法: EM (サブトモグラム平均) / 解像度: 7.3 Å EMDB-3872: Cryo-electron tomogram of Ebola virus virus-like particles 手法: EM (トモグラフィー) EMDB-3873: The structure of Ebola virus nucleocapsid from virions 手法: EM (サブトモグラム平均) / 解像度: 9.1 Å EMDB-3874: Cryo-electron tomogram of Ebola virus 手法: EM (トモグラフィー) EMDB-3875: The structure of Marburg virus nucleocapsid from virions 手法: EM (サブトモグラム平均) / 解像度: 9.1 Å EMDB-3876: Cryo-electron tomogram of Marburg virus 手法: EM (トモグラフィー)
由来	zaire ebolavirus (strain mayinga-76) (エボラウイルス) Ebola virus - Mayinga, Zaire, 1976 (エボラウイルス) Marburg virus - Musoke, Kenya, 1980 (ウイルス) Marburg virus - Angola (ウイルス)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / nucleocapsid (カプシド) / virus-like particle (ウイルス様粒子)