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Structure paper

タイトル	Katanin spiral and ring structures shed light on power stroke for microtubule severing.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 24, Issue 9, Page 717-725, Year 2017
掲載日	2017年8月7日
著者	Elena Zehr / Agnieszka Szyk / Grzegorz Piszczek / Ewa Szczesna / Xiaobing Zuo / Antonina Roll-Mecak /
PubMed 要旨	Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack ...Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of known 3D structures for these enzymes, their mechanism of action has remained poorly understood. Here we report the X-ray crystal structure of the monomeric AAA katanin module from Caenorhabditis elegans and cryo-EM reconstructions of the hexamer in two conformations. The structures reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to microtubule-severing enzymes and critical for their function. The reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes interprotomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.
リンク	Nat Struct Mol Biol / PubMed:28783150 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.3 - 6.0 Å
構造データ	8794 5wc0 EMDB-8794, PDB-5wc0: katanin hexamer in spiral conformation 手法: EM (単粒子) / 解像度: 4.4 Å 8796 5wcb EMDB-8796, PDB-5wcb: Katanin hexamer in the ring conformation 手法: EM (単粒子) / 解像度: 6.0 Å PDB-5wc1: katanin AAA ATPase domain 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-SO4*: SULFATE ION / 硫酸ジアニオン / 硫酸塩
由来	caenorhabditis elegans (センチュウ)
キーワード	MOTOR PROTEIN (モータータンパク質) / Microtubule cytoskeleton / microtubule-severing enzyme / AAA ATPase / p60 / CYTOSOLIC PROTEIN (細胞質基質) / microtubule severing enzyme / microtubule severing protein