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Structure paper

タイトル	Architectures of Lipid Transport Systems for the Bacterial Outer Membrane.
ジャーナル・号・ページ	Cell, Vol. 169, Issue 2, Page 273-285.e17, Year 2017
掲載日	2017年4月6日
著者	Damian C Ekiert / Gira Bhabha / Georgia L Isom / Garrett Greenan / Sergey Ovchinnikov / Ian R Henderson / Jeffery S Cox / Ronald D Vale /
PubMed 要旨	How phospholipids are trafficked between the bacterial inner and outer membranes through the hydrophilic space of the periplasm is not known. We report that members of the mammalian cell entry (MCE) ...How phospholipids are trafficked between the bacterial inner and outer membranes through the hydrophilic space of the periplasm is not known. We report that members of the mammalian cell entry (MCE) protein family form hexameric assemblies with a central channel capable of mediating lipid transport. The E. coli MCE protein, MlaD, forms a ring associated with an ABC transporter complex in the inner membrane. A soluble lipid-binding protein, MlaC, ferries lipids between MlaD and an outer membrane protein complex. In contrast, EM structures of two other E. coli MCE proteins show that YebT forms an elongated tube consisting of seven stacked MCE rings, and PqiB adopts a syringe-like architecture. Both YebT and PqiB create channels of sufficient length to span the periplasmic space. This work reveals diverse architectures of highly conserved protein-based channels implicated in the transport of lipids between the membranes of bacteria and some eukaryotic organelles.
リンク	Cell / PubMed:28388411 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.501 - 25.0 Å
構造データ	8608 5uvn EMDB-8608, PDB-5uvn: Structure of E. coli MCE protein PqiB, periplasmic domain 手法: EM (単粒子) / 解像度: 3.96 Å EMDB-8610: Structure of E. coli MCE protein PqiB, periplasmic domain 手法: EM (単粒子) / 解像度: 10.0 Å EMDB-8611: Negative stain reconstruction of E. coli MCE protein YebT 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-8612: Negative stain reconstruction of E. coli MCE protein PqiB 手法: EM (単粒子) / 解像度: 25.0 Å PDB-5uw2: Structure of E. coli MCE protein MlaD, periplasmic domain 手法: X-RAY DIFFRACTION / 解像度: 2.85 Å PDB-5uw8: Structure of E. coli MCE protein MlaD, core MCE domain 手法: X-RAY DIFFRACTION / 解像度: 2.15 Å PDB-5uwa: Structure of E. coli phospholipid binding protein MlaC 手法: X-RAY DIFFRACTION / 解像度: 1.501 Å PDB-5uwb: Re-refined 4FCZ: lipid-bound crystal structure of toluene-tolerance protein from Pseudomonas putida 手法: X-RAY DIFFRACTION / 解像度: 2.604 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-HOH: WATER / 水 ChemComp-8ND: (2S)-3-(2-aminoethoxy)propane-1,2-diyl dihexadecanoate ChemComp-PEF: DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / DPPE / リン脂質*YM / ホスファチジルエタノールアミン
由来	escherichia coli (大腸菌) escherichia coli o157:h7 (大腸菌) escherichia coli (strain k12) (大腸菌) pseudomonas putida (strain atcc 47054 / dsm 6125 / ncimb 11950 / kt2440) (シュードモナス・プチダ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / MCE protein / bacterial lipid transport / phospholipid-binding protein / Lipid-binding / periplasmic (ペリプラズム) / MlaC / transport