Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Atomic structures of an entire contractile injection system in both the extended and contracted states.
Journal, issue, pages	Nat Microbiol, Vol. 4, Issue 11, Page 1885-1894, Year 2019
Publish date	Aug 5, 2019
Authors	Ambroise Desfosses / Hariprasad Venugopal / Tapan Joshi / Jan Felix / Matthew Jessop / Hyengseop Jeong / Jaekyung Hyun / J Bernard Heymann / Mark R H Hurst / Irina Gutsche / Alok K Mitra /
PubMed Abstract	Contractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, ...Contractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, bacterial type six secretion systems and R-pyocins is rapidly increasing, structural information on the contraction of bacterial phage-like protein-translocation structures directed towards eukaryotic hosts is scarce. Here, we characterize the antifeeding prophage AFP from Serratia entomophila by cryo-electron microscopy. We present the high-resolution structure of the entire AFP particle in the extended state, trace 11 protein chains de novo from the apical cap to the needle tip, describe localization variants and perform specific structural comparisons with related systems. We analyse inter-subunit interactions and highlight their universal conservation within contractile injection systems while revealing the specificities of AFP. Furthermore, we provide the structure of the AFP sheath-baseplate complex in a contracted state. This study reveals atomic details of interaction networks that accompany and define the contraction mechanism of toxin-delivery tailocins, offering a comprehensive framework for understanding their mode of action and for their possible adaptation as biocontrol agents.
External links	Nat Microbiol / PubMed:31384001 / PubMed Central
Methods	EM (single particle) / EM (helical sym.)
Resolution	2.8 - 5.4 Å
Structure data	4782 6rao EMDB-4782, PDB-6rao: Cryo-EM structure of the anti-feeding prophage (AFP) baseplate, 6-fold symmetrised Method: EM (single particle) / Resolution: 3.1 Å EMDB-4783: Composite map of an entire contractile injection device : the anti-feeding prophage (AFP) Method: EM (single particle) / Resolution: 3.2 Å 4784 6rap EMDB-4784, PDB-6rap: Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap) Method: EM (single particle) / Resolution: 3.3 Å 4800 6rbk EMDB-4800, PDB-6rbk: Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in extended state, 3-fold symmetrised Method: EM (single particle) / Resolution: 3.4 Å EMDB-4801: Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap), ending with Afp3 Method: EM (single particle) / Resolution: 3.4 Å 4802 6rbn EMDB-4802, PDB-6rbn: Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath-tube complex in extended state Method: EM (helical sym.) / Resolution: 2.8 Å 4803 6rc8 EMDB-4803, PDB-6rc8: Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath in contracted state Method: EM (helical sym.) / Resolution: 3.8 Å EMDB-4859: Cryo-EM structure of the anti-feeding prophage (AFP) sheath-tube in contracted state, C6 symmetrized Method: EM (single particle) / Resolution: 4.2 Å EMDB-4871: Cryo-EM structure of the anti-feeding prophage (AFP) needle from signal-subtracted particles Method: EM (single particle) / Resolution: 3.7 Å 4876 6rgl EMDB-4876, PDB-6rgl: Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in contracted state Method: EM (single particle) / Resolution: 5.4 Å
Source	serratia entomophila (bacteria)
Keywords	VIRUS LIKE PARTICLE / Anti-feeding prophage / secretion system / AFP / contractile / cap / tube terminating protein / baseplate / sheath / tube / contracted