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TitleCapsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 116, Issue 42, Page 21037-21046, Year 2019
Publish dateOct 15, 2019
AuthorsAlexis Huet / Robert L Duda / Pascale Boulanger / James F Conway /
PubMed AbstractThe large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a ...The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a regulated process that involves several intermediates, including a thick-walled round precursor prohead that expands as the viral DNA is packaged to yield a thin-walled and angular mature capsid. We investigated capsid maturation by comparing cryoelectron microscopy (cryo-EM) structures of the prohead, the empty expanded capsid both with and without decoration protein, and the virion capsid at a resolution of 3.8 Å for the latter. We detail the molecular structure of the mcp, its complex pattern of interactions, and their evolution during maturation. The bacteriophage T5 mcp is a variant of the canonical HK97-fold with a high level of plasticity that allows for the precise assembly of a giant macromolecule and the adaptability needed to interact with other proteins and the packaged DNA.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:31578255 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 7.2 Å
Structure data

EMDB-20099, PDB-6okb:
Prohead 2 of the phage T5
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-20122, PDB-6oma:
non-decorated head of the phage T5
Method: EM (single particle) / Resolution: 7.2 Å

EMDB-20123:
decorated head of the phage T5
Method: EM (single particle) / Resolution: 6.8 Å

EMDB-20125, PDB-6omc:
capsid of T5 virion
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • escherichia phage t5 (bacteriophage)
KeywordsVIRUS LIKE PARTICLE / procapsid / HK97-fold / dsDNA-phage / icosahedral / VIRUS / capsid

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