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TitleMitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Journal, issue, pagesScience, Vol. 365, Issue 6458, Page 1144-1149, Year 2019
Publish dateSep 13, 2019
AuthorsMartin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / Daniel Boehringer / André Schneider / Nenad Ban /
PubMed AbstractMitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome ...Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.
External linksPubMed:31515389 / Publisher's page
KeywordsRIBOSOME / mitochondrial ribosome / assembly intermediate / translation
MethodsEM (single particle)
Resolution3.1 - 5.9 A
Structure data

EMDB-10175:
Head domain of the mt-SSU assemblosome from Trypanosoma brucei

EMDB-10177:
Body domain of the mt-SSU assemblosome from Trypanosoma brucei

PDB-6sg9:
Head domain of the mt-SSU assemblosome from Trypanosoma brucei

PDB-6sga:
Body domain of the mt-SSU assemblosome from Trypanosoma brucei

EMDB-10176:
mt-SSU assemblosome of wild-type Trypanosoma brucei brucei

EMDB-10178:
mt-SSU middle assembly intermediate of wild-type Trypanosoma brucei brucei

EMDB-10179:
mt-SSU late assembly intermediate of wild-type Trypanosoma brucei brucei

EMDB-10180:
mt-SSU assemblosome of Trypanosoma brucei

PDB-6sgb:
mt-SSU assemblosome of Trypanosoma brucei

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate

ChemComp-MG:
MAGNESIUM IONMagnesium

ChemComp-ZN:
ZINC IONZinc

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINES-Adenosyl-L-homocysteine

ChemComp-HOH:
WATERWater

ChemComp-PO4:
PHOSPHATE IONPhosphate

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

Source
  • trypanosoma brucei brucei (eukaryote)
  • Trypanosoma brucei brucei

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