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TitleMitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Journal, issue, pagesScience, Vol. 365, Issue 6458, Page 1144-1149, Year 2019
Publish dateSep 13, 2019
AuthorsMartin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / Daniel Boehringer / André Schneider / Nenad Ban /
PubMed AbstractMitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome ...Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.
External linksScience / PubMed:31515389
MethodsEM (single particle)
Resolution3.1 - 5.9 Å
Structure data

EMDB-10175, PDB-6sg9:
Head domain of the mt-SSU assemblosome from Trypanosoma brucei
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-10176:
mt-SSU assemblosome of wild-type Trypanosoma brucei brucei
Method: EM (single particle) / Resolution: 4 Å

EMDB-10177, PDB-6sga:
Body domain of the mt-SSU assemblosome from Trypanosoma brucei
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-10178:
mt-SSU middle assembly intermediate of wild-type Trypanosoma brucei brucei
Method: EM (single particle) / Resolution: 5.2 Å

EMDB-10179:
mt-SSU late assembly intermediate of wild-type Trypanosoma brucei brucei
Method: EM (single particle) / Resolution: 5.9 Å

EMDB-10180, PDB-6sgb:
mt-SSU assemblosome of Trypanosoma brucei
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
MAGNESIUM ION / Magnesium

ChemComp-ZN:
ZINC ION / Zinc

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE / S-Adenosyl-L-homocysteine

ChemComp-HOH:
WATER / Water

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

Source
  • trypanosoma brucei brucei (eukaryote)
  • Trypanosoma brucei brucei
KeywordsCryoelectron Microscopy / Mitochondrial Proteins / Mitochondrial Ribosomes / Models, Molecular / Nucleic Acid Conformation / Protein Structure, Quaternary / RNA Interference / RNA Stability / RNA, Ribosomal / Ribosomal Proteins / Trypanosoma brucei brucei / RIBOSOME / mitochondrial ribosome / assembly intermediate / translation

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