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TitleElectron cryo-microscopy of bacteriophage PR772 reveals the elusive vertex complex and the capsid architecture.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateSep 12, 2019
AuthorsHemanth Kn Reddy / Marta Carroni / Janos Hajdu / Martin Svenda /
PubMed AbstractBacteriophage PR772, a member of the family, has a 70 nm diameter icosahedral protein capsid that encapsulates a lipid membrane, dsDNA, and various internal proteins. An icosahedrally averaged ...Bacteriophage PR772, a member of the family, has a 70 nm diameter icosahedral protein capsid that encapsulates a lipid membrane, dsDNA, and various internal proteins. An icosahedrally averaged CryoEM reconstruction of the wild-type virion and a localized reconstruction of the vertex region reveal the composition and the structure of the vertex complex along with new protein conformations that play a vital role in maintaining the capsid architecture of the virion. The overall resolution of the virion is 2.75 Å, while the resolution of the protein capsid is 2.3 Å. The conventional penta-symmetron formed by the capsomeres is replaced by a large vertex complex in the pseudo T = 25 capsid. All the vertices contain the host-recognition protein, P5; two of these vertices show the presence of the receptor-binding protein, P2. The 3D structure of the vertex complex shows interactions with the viral membrane, indicating a possible mechanism for viral infection.
External linksElife / PubMed:31513011 / PubMed Central
MethodsEM (single particle)
Resolution2.75 - 10 Å
Structure data

EMDB-10237:
Localized Reconstruction of the vertex region of Bacteriophage PR772 showing the heteropentameric penton
Method: EM (single particle) / Resolution: 4.41 Å

EMDB-10238:
Focused Classification of the vertex region of Bacteriophage PR772 showing the heteropentameric penton
Method: EM (single particle) / Resolution: 4.25 Å

EMDB-4461, PDB-6q5u:
High resolution electron cryo-microscopy structure of the bacteriophage PR772
Method: EM (single particle) / Resolution: 2.75 Å

EMDB-4462:
Vertex Complex of Bacteriophage PR772
Method: EM (single particle) / Resolution: 10 Å

Source
  • enterobacteria phage pr772 (virus)
KeywordsBacteriophages / Capsid / Capsid Proteins / Cryoelectron Microscopy / Image Processing, Computer-Assisted / Protein Conformation / Tectiviridae / VIRUS / Phage / Membrane / double-barrel / helix turn helix / helix with a kink / beta-propeller / heteropentamer / penton

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