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TitleAtomic structures of an entire contractile injection system in both the extended and contracted states.
Journal, issue, pagesNat Microbiol, Year 2019
Publish dateAug 5, 2019
AuthorsAmbroise Desfosses / Hariprasad Venugopal / Tapan Joshi / Jan Felix / Matthew Jessop / Hyengseop Jeong / Jaekyung Hyun / J Bernard Heymann / Mark R H Hurst / Irina Gutsche / Alok K Mitra /
PubMed AbstractContractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, ...Contractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, bacterial type six secretion systems and R-pyocins is rapidly increasing, structural information on the contraction of bacterial phage-like protein-translocation structures directed towards eukaryotic hosts is scarce. Here, we characterize the antifeeding prophage AFP from Serratia entomophila by cryo-electron microscopy. We present the high-resolution structure of the entire AFP particle in the extended state, trace 11 protein chains de novo from the apical cap to the needle tip, describe localization variants and perform specific structural comparisons with related systems. We analyse inter-subunit interactions and highlight their universal conservation within contractile injection systems while revealing the specificities of AFP. Furthermore, we provide the structure of the AFP sheath-baseplate complex in a contracted state. This study reveals atomic details of interaction networks that accompany and define the contraction mechanism of toxin-delivery tailocins, offering a comprehensive framework for understanding their mode of action and for their possible adaptation as biocontrol agents.
External linksPubMed:31384001 / Publisher's page
KeywordsVIRUS LIKE PARTICLE / Anti-feeding prophage / secretion system / AFP / contractile / cap / tube terminating protein / baseplate / sheath / tube / contracted
MethodsEM (single particle) / EM (helical sym.)
Resolution2.8 - 5.4 A
Structure data

EMDB-4782:
Cryo-EM structure of the anti-feeding prophage (AFP) baseplate, 6-fold symmetrised

EMDB-4783:
Composite map of an entire contractile injection device : the anti-feeding prophage (AFP)

EMDB-4784:
Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap)

EMDB-4800:
Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in extended state, 3-fold symmetrised

EMDB-4801:
Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap), ending with Afp3

EMDB-4802:
Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath-tube complex in extended state

EMDB-4803:
Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath in contracted state

EMDB-4859:
Cryo-EM structure of the anti-feeding prophage (AFP) sheath-tube in contracted state, C6 symmetrized

EMDB-4871:
Cryo-EM structure of the anti-feeding prophage (AFP) needle from signal-subtracted particles

EMDB-4876:
Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in contracted state

PDB-6rao:
Cryo-EM structure of the anti-feeding prophage (AFP) baseplate, 6-fold symmetrised

PDB-6rap:
Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap)

PDB-6rbk:
Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in extended state, 3-fold symmetrised

PDB-6rbn:
Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath-tube complex in extended state

PDB-6rc8:
Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath in contracted state

PDB-6rgl:
Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in contracted state

Source
  • serratia entomophila (bacteria)

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