|Title||Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes.|
|Journal, issue, pages||Science, Vol. 364, Issue 6440, Page 552-557, Year 2019|
|Publish date||May 10, 2019|
|Authors||Shoji Maeda / Qianhui Qu / Michael J Robertson / Georgios Skiniotis / Brian K Kobilka /|
|PubMed Abstract||Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes ...Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes (M1R to M5R), which, despite sharing a high degree of sequence identity in the transmembrane region, couple to different heterotrimeric GTP-binding proteins (G proteins) to transmit signals. M1R, M3R, and M5R couple to the G family, whereas M2R and M4R couple to the G family. Here, we present and compare the cryo-electron microscopy structures of M1R in complex with G and M2R in complex with G The M1R-G complex exhibits distinct features, including an extended transmembrane helix 5 and carboxyl-terminal receptor tail that interacts with G protein. Detailed analysis of these structures provides a framework for understanding the molecular determinants of G-protein coupling selectivity.|
|External links||PubMed:31073061 / Publisher's page|
|Keywords||SIGNALING PROTEIN / G-protein coupled receptor-G-protein complex / neurotransmitter receptor|
|Methods||EM (single particle)|
|Resolution||3.3 - 3.6 A|
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