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Structure paper

TitleEnterovirus particles expel capsid pentamers to enable genome release.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 1138-1138, Year 2019
Publish dateMar 8, 2019
AuthorsDavid Buchta / Tibor Füzik / Dominik Hrebík / Yevgen Levdansky / Lukáš Sukeník / Liya Mukhamedova / Jana Moravcová / Robert Vácha / Pavel Plevka /
PubMed AbstractViruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for ...Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 Å in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
External linksPubMed:30850609 / Publisher's page
KeywordsAnimals / Capsid / Cercopithecus aethiops / Cryoelectron Microscopy / Enterovirus B, Human / Epithelial Cells / Genome, Viral / Humans / Hydrogen-Ion Concentration / Molecular Dynamics Simulation / RNA, Double-Stranded / RNA, Viral / Virion / Virus Uncoating / VIRUS / echovirus / echovirus 18 / native particle / enterovirus / picornavirus / altered particle / A-particle / empty particle / B-particle / open particle / O-particle / genome release
MethodsEM (single particle, icos. sym.) / EM (single particle)
Resolution3.16 - 19.4 A
Structure data

EMDB-0181:
Echovirus 18 native particle

EMDB-0185:
Echovirus 18 Open particle without one pentamer

EMDB-0188:
Echovirus 18 Open particle without three pentamers

EMDB-0184:
Echovirus 18 Open particle without one pentamer

EMDB-0186:
Echovirus 18 Open particle without two pentamers

EMDB-0187:
Echovirus 18 Open particle without three pentamers

EMDB-0189:
Echovirus 30 Open particle without one pentamer

EMDB-0182:
Echovirus 18 A-particle

EMDB-0217:
Echovirus 18 Open particle without two pentamers

EMDB-0183:
Echovirus 18 empty particle

PDB-6hbg:
Echovirus 18 native particle

PDB-6hbh:
Echovirus 18 A-particle

PDB-6hbj:
Echovirus 18 empty particle

PDB-6hbk:
Echovirus 18 Open particle without one pentamer

PDB-6hbl:
Echovirus 18 Open particle without three pentamers

PDB-6hht:
Echovirus 18 Open particle without two pentamers

Chemicals

ChemComp-PLM:
PALMITIC ACIDPalmitic acid

ChemComp-GUN:
GUANINEGuanine

Source
  • echovirus e18
  • Echovirus E30

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