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Structure paper

TitleHigh-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 1032-1032, Year 2019
Publish dateMar 4, 2019
AuthorsMark A Herzik / Mengyu Wu / Gabriel C Lander
External linksPubMed:30833564 / Publisher's page
KeywordsOXYGEN TRANSPORT / heme-binding / hetero-4-mer / globin / OXIDOREDUCTASE / dehydrogenase / NADH-binding / homo-2-mer
MethodsEM (single particle)
Resolution2.8 - 6 A
Structure data

EMDB-0408:
Single-particle cryo-EM reconstruction of human methemoglobin using 200 keV, state 2

EMDB-0409:
Single-particle cryo-EM reconstruction of the catalytic subunit of protein kinase A bound to ATP and IP20 using 200 keV

PDB-6nbd:
Human methemoglobin state 2 determined using single-particle cryo-EM at 200 keV

PDB-6nbb:
Horse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keV

PDB-6nbc:
human methemoglobin state 1 determined using single-particle cryo-EM at 200 keV

EMDB-0406:
Single-particle cryo-EM reconstruction of horse liver alcohol dehydrogenase using 200 keV

EMDB-0407:
Single-particle cryo-EM reconstruction of human methemoglobin using 200 keV, state 1

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FEHeme

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDENicotinamide adenine dinucleotide

ChemComp-ZN:
ZINC IONZinc

Source
  • Homo sapiens (human)
  • Human (human)
  • Mus musculus (house mouse)
  • homo sapiens (human)
  • equus caballus (horse)
  • Equus caballus (horse)

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    • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
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