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Structure paper

TitleCryo-EM Structure of the Human Ribonuclease P Holoenzyme.
Journal, issue, pagesCell, Vol. 175, Issue 5, Page 1393-1404.e11, Year 2018
Publish dateNov 15, 2018
AuthorsJian Wu / Shuangshuang Niu / Ming Tan / Chenhui Huang / Mingyue Li / Yang Song / Qianmin Wang / Juan Chen / Shaohua Shi / Pengfei Lan / Ming Lei
External linksPubMed:30454648 / Publisher's page
KeywordsHYDROLASE/RNA / Ribonuclease P / RNA-protein complex / HYDROLASE-RNA complex / HYDROLASE / RNase P
MethodsEM (single particle) / X-ray diffraction
Resolution2.6 - 3.92 A
Structure data

EMDB-9626:
Cryo-EM structure of human Ribonuclease P

EMDB-9627:
Cryo-EM structure of human Ribonuclease P with mature tRNA

PDB-6ahr:
Cryo-EM structure of human Ribonuclease P

PDB-6ahu:
Cryo-EM structure of human Ribonuclease P with mature tRNA

PDB-6ahv:
Crystal structure of human RPP40

Chemicals

ChemComp-ZN:
ZINC IONZinc

ChemComp-HOH:
WATERWater

Source
  • Homo sapiens (human)
  • Human (human)
  • homo sapiens (human)

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Feb 20, 2018. PDBj/BINDS workshop in Osaka University

PDBj/BINDS workshop in Osaka University

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    Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

    Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

    • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
    • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
    • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

    External links: The 2017 Nobel Prize in Chemistry - Press Release

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    Jul 12, 2017. Major update of PDB

    Major update of PDB

    • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary. This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated. See below links for details.
    • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software). Now, EM Navigator and Yorodumi are based on the updated data.

    External links: wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

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    EMN Papers

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