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Structure paper

TitleStructural insight into precursor tRNA processing by yeast ribonuclease P.
Journal, issue, pagesScience, Vol. 362, Issue 6415, Year 2018
Publish dateSep 27, 2018
AuthorsPengfei Lan / Ming Tan / Yuebin Zhang / Shuangshuang Niu / Juan Chen / Shaohua Shi / Shuwan Qiu / Xuejuan Wang / Xiangda Peng / Gang Cai / Hong Cheng / Jian Wu / Guohui Li / Ming Lei
External linksPubMed:30262633 / Publisher's page
KeywordsHYDROLASE/RNA / Ribonuclease P / RNA-protein complex / HYDROLASE-RNA complex
MethodsEM (single particle)
Resolution3.48 A
Structure data

EMDB-9616:
Cryo-EM structure of yeast Ribonuclease P

EMDB-9622:
Cryo-EM structure of yeast Ribonuclease P with pre-tRNA substrate

PDB-6agb:
Cryo-EM structure of yeast Ribonuclease P

PDB-6ah3:
Cryo-EM structure of yeast Ribonuclease P with pre-tRNA substrate

Chemicals

ChemComp-ZN:
ZINC IONZinc

ChemComp-MG:
MAGNESIUM IONMagnesium

Source
  • Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
  • Baker's yeast (baker's yeast)
  • Saccharomyces cerevisiae S288c (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae s288c (yeast)

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    • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
    • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
    • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

    External links: The 2017 Nobel Prize in Chemistry - Press Release

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