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| Title | Structure and mechanism of the plant RNA polymerase V. |
|---|---|
| Journal, issue, pages | Science, Vol. 379, Issue 6638, Page 1209-1213, Year 2023 |
| Publish date | Mar 24, 2023 |
 Authors | Guohui Xie / Xuan Du / Hongmiao Hu / Sisi Li / Xiaofeng Cao / Steven E Jacobsen / Jiamu Du /   |
| PubMed Abstract | In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation ...In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage, which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation. |
 External links | Science / PubMed:36893216 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 2.8 - 3.57 Å |
| Structure data | EMDB-34820, PDB-8hil: EMDB-34821, PDB-8him: |
| Chemicals |  ChemComp-ZN:  ChemComp-MG: |
| Source |
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 Keywords |  TRANSCRIPTION / DNA-dependent RNA polymerase V |
