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TitleCryo-EM structures of thylakoid-located voltage-dependent chloride channel VCCN1.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 2505, Year 2022
Publish dateMay 6, 2022
AuthorsTatsuya Hagino / Takafumi Kato / Go Kasuya / Kan Kobayashi / Tsukasa Kusakizako / Shin Hamamoto / Tomoaki Sobajima / Yuichiro Fujiwara / Keitaro Yamashita / Hisashi Kawasaki / Andrés D Maturana / Tomohiro Nishizawa / Osamu Nureki /
PubMed AbstractIn the light reaction of plant photosynthesis, modulation of electron transport chain reactions is important to maintain the efficiency of photosynthesis under a broad range of light intensities. ...In the light reaction of plant photosynthesis, modulation of electron transport chain reactions is important to maintain the efficiency of photosynthesis under a broad range of light intensities. VCCN1 was recently identified as a voltage-gated chloride channel residing in the thylakoid membrane, where it plays a key role in photoreaction tuning to avoid the generation of reactive oxygen species (ROS). Here, we present the cryo-EM structures of Malus domestica VCCN1 (MdVCCN1) in nanodiscs and detergent at 2.7 Å and 3.0 Å resolutions, respectively, and the structure-based electrophysiological analyses. VCCN1 structurally resembles its animal homolog, bestrophin, a Ca-gated anion channel. However, unlike bestrophin channels, VCCN1 lacks the Ca-binding motif but instead contains an N-terminal charged helix that is anchored to the lipid membrane through an additional amphipathic helix. Electrophysiological experiments demonstrate that these structural elements are essential for the channel activity, thus revealing the distinct activation mechanism of VCCN1.
External linksNat Commun / PubMed:35523970 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 3.0 Å
Structure data

EMDB-31165, PDB-7ek1:
Cryo-EM structure of VCCN1 in detergent
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-31166, PDB-7ek2:
Cryo-EM structure of VCCN1 in lipid nanodisc
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-31167, PDB-7ek3:
Cryo-EM structure of VCCN1 Y332A mutant in lipid nanodisc
Method: EM (single particle) / Resolution: 2.7 Å

Source
  • malus domestica (apple)
KeywordsMEMBRANE PROTEIN / Ion channel / Thylakoid / Photosynthesis / Bestrophin family

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