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TitleStructural and functional properties of a magnesium transporter of the SLC11/NRAMP family.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateJan 10, 2022
AuthorsKarthik Ramanadane / Monique S Straub / Raimund Dutzler / Cristina Manatschal /
PubMed AbstractMembers of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca and ...Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca and Mg, which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg. The protein transports Mg and Mn but not Ca by a mechanism that is not coupled to H. Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell.
External linksElife / PubMed:35001872 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.5 - 4.6 Å
Structure data

13985

7qia

EMDB-13985: Cryo-EM map of apo-EleNRMT in complex with two nanobodies at 3.5A
PDB-7qia: Structure of apo-EleNRMT in complex with two nanobodies at 3.5A
Method: EM (single particle) / Resolution: 3.5 Å

13987

7qic

EMDB-13987: Cryo-EM map of magnesium-bound EleNRMT in complex with two nanobodies at 4.1A
PDB-7qic: Structure of magnesium-bound EleNRMT in complex with two nanobodies at 4.1A
Method: EM (single particle) / Resolution: 4.1 Å

PDB-7qji:
X-Ray Structure of apo-EleNRMT in complex with two Nanobodies at 4.1A
Method: X-RAY DIFFRACTION / Resolution: 4.1 Å

PDB-7qjj:
X-Ray Structure of a Mn2+ soak of EleNRMT in complex with two Nanobodies at 4.6A
Method: X-RAY DIFFRACTION / Resolution: 4.6 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-MG:
Unknown entry

ChemComp-MN:
Unknown entry

Source
  • eggerthella lenta (bacteria)
  • synthetic construct (others)
  • vicugna pacos (alpaca)
KeywordsMEMBRANE PROTEIN / SLC11 / Magnesium / LeuT fold / NRAMP-related Mg2+ transporter / Nanobody complex

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