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TitleSingle-particle cryo-EM reveals conformational variability of the oligomeric VCC β-barrel pore in a lipid bilayer.
Journal, issue, pagesJ Cell Biol, Vol. 220, Issue 12, Year 2021
Publish dateDec 6, 2021
AuthorsNayanika Sengupta / Anish Kumar Mondal / Suman Mishra / Kausik Chattopadhyay / Somnath Dutta /
PubMed AbstractVibrio cholerae cytolysin (VCC) is a water-soluble, membrane-damaging, pore-forming toxin (PFT) secreted by pathogenic V. cholerae, which causes eukaryotic cell death by altering the plasma membrane ...Vibrio cholerae cytolysin (VCC) is a water-soluble, membrane-damaging, pore-forming toxin (PFT) secreted by pathogenic V. cholerae, which causes eukaryotic cell death by altering the plasma membrane permeability. VCC self-assembles on the cell surface and undergoes a dramatic conformational change from prepore to heptameric pore structure. Over the past few years, several high-resolution structures of detergent-solubilized PFTs have been characterized. However, high-resolution structural characterization of small β-PFTs in a lipid environment is still rare. Therefore, we used single-particle cryo-EM to characterize the structure of the VCC oligomer in large unilamellar vesicles, which is the first atomic-resolution cryo-EM structure of VCC. From our study, we were able to provide the first documented visualization of the rim domain amino acid residues of VCC interacting with lipid membrane. Furthermore, cryo-EM characterization of lipid bilayer-embedded VCC suggests interesting conformational variabilities, especially in the transmembrane channel, which could have a potential impact on the pore architecture and assist us in understanding the pore formation mechanism.
External linksJ Cell Biol / PubMed:34617964 / PubMed Central
MethodsEM (single particle)
Resolution4.0 - 5.6 Å
Structure data

EMDB-31972:
Cryo-EM 3D reconstruction of Vibrio cholerae Cytolysin embedded in lipid bilayer- State 3
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-31973:
Cryo-EM 3D reconstruction of Vibrio cholerae Cytolysin adhered to liposome membrane surface
Method: EM (single particle) / Resolution: 5.3 Å

EMDB-31974:
Cryo-EM 3D reconstruction of Vibrio cholerae Cytolysin partially embedded in lipid bilayer- State 2
Method: EM (single particle) / Resolution: 5.6 Å

Source
  • Vibrio cholerae (bacteria)

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