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Structure paper

TitleMolecular structure of the intact bacterial flagellar basal body.
Journal, issue, pagesNat Microbiol, Vol. 6, Issue 6, Page 712-721, Year 2021
Publish dateApr 30, 2021
AuthorsSteven Johnson / Emily J Furlong / Justin C Deme / Ashley L Nord / Joseph J E Caesar / Fabienne F V Chevance / Richard M Berry / Kelly T Hughes / Susan M Lea /
PubMed AbstractThe bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the ...The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids. Here, we present structures of the intact Salmonella flagellar basal body, encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 Å. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine.
External linksNat Microbiol / PubMed:33931760 / PubMed Central
MethodsEM (single particle)
Resolution2.2 - 3.8 Å
Structure data

EMDB-12183, PDB-7bgl:
Salmonella LP ring 26 mer refined in C26 map
Method: EM (single particle) / Resolution: 2.2 Å

EMDB-12190, PDB-7bhq:
In situ assembled Salmonella FlgD hook cap complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-12192, PDB-7bin:
Salmonella export gate and rod refined in focussed C1 map
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-12193, PDB-7bj2:
Salmonella flagellar basal body assembly intermediate - P ring alone structure
Method: EM (single particle) / Resolution: 3 Å

EMDB-12195, PDB-7bk0:
Salmonella FliF ring (34mer) in intact basal body - C1
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-12603, PDB-7nvg:
Salmonella flagellar basal body refined in C1 map
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-TLW:
(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4,5-bis(oxidanyl)oxane-2-carboxylic acid

ChemComp-TQN:
[(3~{R})-1-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-3-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-(hydroxymethyl)-5-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]oxymethyl]-5-oxidanyl-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-2-phosphonooxy-oxan-3-yl]amino]-1-oxidanylidene-tetradecan-3-yl] hexadecanoate

Source
  • salmonella enterica subsp. enterica serovar typhi (bacteria)
  • salmonella typhimurium (strain lt2 / sgsc1412 / atcc 700720) (bacteria)
  • salmonella typhi (bacteria)
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsMEMBRANE PROTEIN / bacterial flagellum LP ring salmonella / bacterial flagellum hook cap salmonella flagellar assembly / PROTEIN TRANSPORT / bacterial flagellum rod bacterial flagellum export gate basal body / bacterial flagellum basal body MS-ring / bacterial flagellum / flagella / basal body

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