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Structure paper

TitleSteps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ.
Journal, issue, pagesScience, Vol. 371, Issue 6524, Year 2021
Publish dateJan 1, 2021
AuthorsNelly Said / Tarek Hilal / Nicholas D Sunday / Ajay Khatri / Jörg Bürger / Thorsten Mielke / Georgiy A Belogurov / Bernhard Loll / Ranjan Sen / Irina Artsimovitch / Markus C Wahl /
PubMed AbstractFactor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ...Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ρ on a pathway to terminating NusA/NusG-modified elongation complexes. An open ρ ring contacts NusA, NusG, and multiple regions of RNA polymerase, trapping and locally unwinding proximal upstream DNA. NusA wedges into the ρ ring, initially sequestering RNA. Upon deflection of distal upstream DNA over the RNA polymerase zinc-binding domain, NusA rotates underneath one capping ρ subunit, which subsequently captures RNA. After detachment of NusG and clamp opening, RNA polymerase loses its grip on the RNA:DNA hybrid and is inactivated. Our structural and functional analyses suggest that ρ, and other termination factors across life, may use analogous strategies to allosterically trap transcription complexes in a moribund state.
External linksScience / PubMed:33243850 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 5.7 Å
Structure data

11087

6z9p

EMDB-11087: Transcription termination complex 1
PDB-6z9p: Transcription termination intermediate complex 1
Method: EM (single particle) / Resolution: 3.9 Å

11088

6z9q

EMDB-11088, PDB-6z9q:
Transcription termination intermediate complex 2
Method: EM (single particle) / Resolution: 5.7 Å

11089

6z9r

EMDB-11089, PDB-6z9r:
Transcription termination intermediate complex 3
Method: EM (single particle) / Resolution: 4.1 Å

11090

6z9s

EMDB-11090: Transcription termination interdmediate complex 4
PDB-6z9s: Transcription termination intermediate complex 4
Method: EM (single particle) / Resolution: 4.4 Å

11091

6z9t

EMDB-11091, PDB-6z9t:
Transcription termination intermediate complex 5
Method: EM (single particle) / Resolution: 4.1 Å

11722

7adb

EMDB-11722, PDB-7adb:
Transcription termination intermediate complex 1 delta NusG
Method: EM (single particle) / Resolution: 4.4 Å

11723

7adc

EMDB-11723, PDB-7adc:
Transcription termination intermediate complex 3 delta NusG
Method: EM (single particle) / Resolution: 4.0 Å

11724

7add

EMDB-11724, PDB-7add:
Transcription termination intermediate complex IIIa
Method: EM (single particle) / Resolution: 4.3 Å

11725

7ade

EMDB-11725, PDB-7ade:
Transcription termination complex IVa
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-DG:
2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE / dGMP*YM / Deoxyguanosine monophosphate

ChemComp-HOH:
WATER / Water

ChemComp-DT:
THYMIDINE-5'-MONOPHOSPHATE / dTMP*YM / Thymidine monophosphate

ChemComp-DA:
2'-DEOXYADENOSINE-5'-MONOPHOSPHATE / dAMP*YM / Deoxyadenosine monophosphate

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / Termination / Helicase / RNA Polymerase / Rho

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