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| Title | Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family. |
|---|---|
| Journal, issue, pages | Nat Chem Biol, Vol. 16, Issue 5, Page 564-569, Year 2020 |
| Publish date | Mar 16, 2020 |
 Authors | Alessandro Grinzato / Eaazhisai Kandiah / Chiara Lico / Camilla Betti / Selene Baschieri / Giuseppe Zanotti /   |
| PubMed Abstract | Potato virus X (PVX) is a positive-sense single-stranded RNA (ssRNA) filamentous plant virus belonging to the Alphaflexiviridae family, considered in recent years as a tool for nanotechnology ...Potato virus X (PVX) is a positive-sense single-stranded RNA (ssRNA) filamentous plant virus belonging to the Alphaflexiviridae family, considered in recent years as a tool for nanotechnology applications. We present the cryo-electron microscopy structure of the PVX particle at a resolution of 2.2 Å. The well-defined density of the coat proteins and of the genomic RNA allowed a detailed analysis of protein-RNA interactions, including those mediated by solvent molecules. The particle is formed by repeated segments made of 8.8 coat proteins, forming a left-handed helical structure. The RNA runs in an internal crevice along the virion, packaged in 5-nucleotide repeats in which the first four bases are stacked in the classical way, while the fifth is rotated and nearly perpendicular. The resolution of the structure described here suggests a mechanism for the virion assembly and potentially provides a platform for the rational design of antiviral compounds and for the use of PVX in nanotechnology. |
 External links | Nat Chem Biol / PubMed:32203412 |
| Methods | EM (helical sym.) |
| Resolution | 2.2 Å |
| Structure data | |
| Chemicals |  ChemComp-HOH: |
| Source |
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 Keywords |  VIRUS / Coat Protein / RNA |
