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Structure paper

TitleStructure of the Decorated Ciliary Doublet Microtubule.
Journal, issue, pagesCell, Vol. 179, Issue 4, Page 909-922.e12, Year 2019
Publish dateOct 31, 2019
AuthorsMeisheng Ma / Mihaela Stoyanova / Griffin Rademacher / Susan K Dutcher / Alan Brown / Rui Zhang /
PubMed AbstractThe axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility ...The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components. We use single-particle cryo-electron microscopy to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs). The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility. Our work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.
External linksCell / PubMed:31668805 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 4.0 Å
Structure data

20631

6u42

EMDB-20631: Composite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii
PDB-6u42: Natively decorated ciliary doublet microtubule
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-20632:
Composite cryo-EM density map of half 1 of the 96-nm repeat of the doublet microtubule from wild-type Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-20633:
Composite cryo-EM density map of half 2 of the 96-nm repeat of the doublet microtubule from wild-type Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 3.57 Å

EMDB-20634:
Composite cryo-EM density map of protofilaments A07-A08 of the 48-nm repeat doublet microtubule (extended lattice)
Method: EM (single particle) / Resolution: 3.45 Å

EMDB-20635:
Composite cryo-EM density map of protofilaments A07-A08 of the 48-nm repeat doublet microtubule (compact lattice)
Method: EM (single particle) / Resolution: 3.66 Å

EMDB-20636:
Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Chlamydomonas reinhardtii rib72 mutant
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-20637:
Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Chlamydomonas reinhardtii fap166 mutant
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPROTEIN FIBRIL / motile cilia / microtubule doublet / repetitive structure

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