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Structure paper

TitleStructural basis for the adsorption of a single-stranded RNA bacteriophage.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 3130, Year 2019
Publish dateJul 16, 2019
AuthorsRan Meng / Mengqiu Jiang / Zhicheng Cui / Jeng-Yih Chang / Kailu Yang / Joanita Jakana / Xinzhe Yu / Zhao Wang / Bo Hu / Junjie Zhang /
PubMed AbstractSingle-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of ...Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.
External linksNat Commun / PubMed:31311931 / PubMed Central
MethodsEM (subtomogram averaging) / EM (single particle)
Resolution5.0 - 32.3 Å
Structure data

EMDB-0338:
MS2 / F-pilus complex
Method: EM (subtomogram averaging) / Resolution: 32.3 Å

EMDB-0448:
F-pilus/MS2 complex (Class 2 map with genome masked out)
Method: EM (single particle) / Resolution: 5.6 Å

EMDB-0450:
F-pilus/MS2 complex (Class 3 map with genome masked out)
Method: EM (single particle) / Resolution: 7.3 Å

EMDB-0451:
Enterobacteria phage MS2
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-0453:
F-pilus/MS2 complex (all particles map with genome masked out)
Method: EM (single particle) / Resolution: 5.0 Å

9397

6nm5

EMDB-9397, PDB-6nm5:
F-pilus/MS2 Maturation protein complex
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-9399:
F-pilus/MS2 complex (Class 1 map with genome masked out)
Method: EM (single particle) / Resolution: 5.8 Å

Chemicals

ChemComp-KSV:
(2R)-2,3-dihydroxypropyl ethyl hydrogen (S)-phosphate

Source
  • Enterobacterio phage MS2 (virus)
  • enterobacteria phage ms2 (virus)
  • escherichia coli (E. coli)
KeywordsPROTEIN BINDING / MS2 maturation protein / F-pilus / adsorption complex

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