[English] 日本語
EMN Papers
- Database of articles cited by 3DEM data entries -

+
Search query

Keywords
Author
Journal
IF

-
Structure paper

TitleThe role of tubulin-tubulin lattice contacts in the mechanism of microtubule dynamic instability.
Journal, issue, pagesNat Struct Mol Biol, Vol. 25, Issue 7, Page 607-615, Year 2018
Publish dateJul 2, 2018
AuthorsSzymon W Manka / Carolyn A Moores /
PubMed AbstractMicrotubules form from longitudinally and laterally assembling tubulin α-β dimers. The assembly induces strain in tubulin, resulting in cycles of microtubule catastrophe and regrowth. This 'dynamic ...Microtubules form from longitudinally and laterally assembling tubulin α-β dimers. The assembly induces strain in tubulin, resulting in cycles of microtubule catastrophe and regrowth. This 'dynamic instability' is governed by GTP hydrolysis that renders the microtubule lattice unstable, but it is unclear how. We used a human microtubule nucleating and stabilizing neuronal protein, doublecortin, and high-resolution cryo-EM to capture tubulin's elusive hydrolysis intermediate GDP•Pi state, alongside the prehydrolysis analog GMPCPP state and the posthydrolysis GDP state with and without an anticancer drug, Taxol. GTP hydrolysis to GDP•Pi followed by Pi release constitutes two distinct structural transitions, causing unevenly distributed compressions of tubulin dimers, thereby tightening longitudinal and loosening lateral interdimer contacts. We conclude that microtubule catastrophe is triggered because the lateral contacts can no longer counteract the strain energy stored in the lattice, while reinforcement of the longitudinal contacts may support generation of force.
External linksNat Struct Mol Biol / PubMed:29967541 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 4.4 Å
Structure data

3961

6evw

EMDB-3961, PDB-6evw:
Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin
Method: EM (single particle) / Resolution: 4.4 Å

3962

6evx

EMDB-3962, PDB-6evx:
Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin
Method: EM (single particle) / Resolution: 4.2 Å

3963

6evy

EMDB-3963, PDB-6evy:
Cryo-EM structure of GTPgammaS-microtubule co-polymerised with doublecortin
Method: EM (single particle) / Resolution: 4.4 Å

3964

6evz

EMDB-3964, PDB-6evz:
Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin
Method: EM (single particle) / Resolution: 3.8 Å

3965

6ew0

EMDB-3965, PDB-6ew0:
Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin and supplemented with Taxol
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • sus sucrofa (pig)
  • Pig (pig)
  • sus scrofa (pig)
KeywordsSTRUCTURAL PROTEIN / microtubule / GTPase / tubulin / Taxol

+
About EMN Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels

Nobel Prize for mechanically activated and temperature-gated ion channels

  • The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
  • EM Navigator can help to find cryo-EM structure data by both pioneers.

External links:The Nobel Prize in Physiology or Medicine 2021 - NobelPrize.org / Structure data by Ardem Patapoutian / Structure data by David Julius

+
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jul 5, 2019. Downlodablable text data

Downlodablable text data

Some data of EM Navigator services can be downloaded as text file. Software such as Excel can load the data files.

PageDataFormat
EMN Searchsearch resultCSV, TSV, or JSON
EMN statisticsdata tableCSV or TSV

Related info.:EMN Search / EMN Statistics

-
EMN Papers

Database of articles cited by 3DEM data entries

  • Database of articles cited by 3DEM data entries in EMDB and PDB
  • Using PubMed data

Related info.:EMDB / PDB / Q: What are the data sources of EM Navigator? / EM Navigator / Yorodumi Papers / Changes in new EM Navigator and Yorodumi

Read more