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TitleFolding DNA into a Lipid-Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins.
Journal, issue, pagesAngew Chem Int Ed Engl, Vol. 57, Issue 8, Page 2072-2076, Year 2018
Publish dateFeb 19, 2018
AuthorsYuanchen Dong / Shuobing Chen / Shijian Zhang / Joseph Sodroski / Zhongqiang Yang / Dongsheng Liu / Youdong Mao /
PubMed AbstractBuilding upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared ...Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared with the membrane-scaffolding-protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins.
External linksAngew Chem Int Ed Engl / PubMed:29266648 / PubMed Central
MethodsEM (single particle)
Resolution7.5 Å
Structure data

7304

6by7

EMDB-7304, PDB-6by7:
Folding DNA into a lipid-conjugated nano-barrel for controlled reconstitution of membrane proteins
Method: EM (single particle) / Resolution: 7.5 Å

Source
  • synthetic construct (others)
KeywordsDNA / DNA nano-barrel DNA origami DNA assembly

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