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Structure paper

TitleDesign of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.
Journal, issue, pagesNat. Biotechnol., Vol. 35, Issue 11, Page 1094-1101, Year 2017
Publish dateOct 16, 2017
AuthorsAjasja Ljubetič / Fabio Lapenta / Helena Gradišar / Igor Drobnak / Jana Aupič / Žiga Strmšek / Duško Lainšček / Iva Hafner-Bratkovič / Andreja Majerle / Nuša Krivec / Mojca Benčina / Tomaž Pisanski / Tanja Ćirković Veličković / Adam Round / José María Carazo / Roberto Melero / Roman Jerala
External linksPubMed:29035374 / Publisher's page
MethodsEM (single particle) / SAS (X-ray synchrotron)
Resolution19 - 28 A
Structure data

EMDB-3781:
TET12SN density map (Design of in vivo self-assembling coiled-coil protein origami)

EMDB-3788:
PYR16SN density map (Design of in vivo self-assembling coiled-coil protein origami)

EMDB-3789:
TRIP18SN density map (Design of in vivo self-assembling coiled-coil protein origami)

SASDCR7:
TET12(1.10)SN-c6

SASDCS7:
TET12(1.10)SN-f9

SASDCT7:
PYR16(4.6)SN-f5

SASDCU7:
TRIP18(7.5R)SN-f5 (TRIP18SN)

SASDCV7:
TET12(2.3)SN-f5b

SASDCW7:
TET12(1.10)S-f5 (TET12S)

SASDCX7:
TET12(1.10)S-f5b

SASDCY7:
TET12(1.10)S-c6

SASDCZ7:
TET12(1.6)S-c6b

SourceDesigned protein
Synthetic construct

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Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

  • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
  • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
  • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

External links: The 2017 Nobel Prize in Chemistry - Press Release

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