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Structure paper

Title3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 24, Issue 11, Page 954-964, Year 2017
Publish dateOct 2, 2017
AuthorsJingdong Cheng / Nikola Kellner / Otto Berninghausen / Ed Hurt / Roland Beckmann
External linksPubMed:28967883 / Publisher's page
MethodsEM (single particle)
Resolution3.2 A
Structure data

EMDB-3847:
Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum

PDB-5oql:
Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum

Chemicals

ChemComp-ZN:
ZINC ION

SourceChaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) / fungus
KeywordsChaetomium / Cryoelectron Microscopy / DNA, Ribosomal / Fungal Proteins / RNA Precursors / RNA, Ribosomal, 18S / Ribosome Subunits, Small, Eukaryotic / RIBOSOME / Ribosome biogenesis

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Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

  • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
  • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
  • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

External links: The 2017 Nobel Prize in Chemistry - Press Release

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Major update of PDB

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EMN Papers

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