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Structure paper

TitleStructures of the CRISPR genome integration complex.
Journal, issue, pagesScience, Vol. 357, Issue 6356, Page 1113-1118, Year 2017
Publish dateJul 20, 2017
AuthorsAddison V Wright / Jun-Jie Liu / Gavin J Knott / Kevin W Doxzen / Eva Nogales / Jennifer A Doudna
External linksPubMed:28729350 / Publisher's page
KeywordsCRISPR-Associated Proteins / CRISPR-Cas Systems / Cas2 protein, E coli / Catalytic Domain / Clustered Regularly Interspaced Short Palindromic Repeats / Cryoelectron Microscopy / Crystallography, X-Ray / DNA, Bacterial / Endodeoxyribonucleases / Endonucleases / Escherichia coli Proteins / Integrases / Integration Host Factors / Mutation / YgbT protein, E coli / integration host factor, E coli / HYDROLASE/DNA / Complex / DNA / HYDROLASE-DNA complex / DNA BINDING PROTEIN/DNA / CRISPR integration complex / Cas1-Cas2 / IHF / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex
MethodsEM (single particle) / X-ray diffraction
Resolution2.9 - 3.89 A
Structure data

EMDB-8827:
Cas1-Cas2-IHF-DNA holo-complex

PDB-5vvj:
Cas1-Cas2 bound to half-site intermediate

PDB-5vvk:
Cas1-Cas2 bound to full-site mimic

PDB-5vvl:
Cas1-Cas2 bound to full-site mimic with Ni

PDB-5wfe:
Cas1-Cas2-IHF-DNA holo-complex

Chemicals

ChemComp-NI:
NICKEL (II) IONNickel

Source
  • Escherichia coli (E. coli)
  • escherichia coli (strain k12) (bacteria)
  • synthetic construct (others)
  • escherichia coli k-12 (bacteria)
  • escherichia coli s88 (bacteria)

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    • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
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    • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

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