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Structure paper

TitleStructure of a pre-catalytic spliceosome.
Journal, issue, pagesNature, Vol. 546, Issue 7660, Page 617-621, Year 2017
Publish dateJun 29, 2017
AuthorsClemens Plaschka / Pei-Chun Lin / Kiyoshi Nagai /
PubMed AbstractIntron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy ...Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy structure of the yeast Saccharomyces cerevisiae pre-catalytic B complex spliceosome at near-atomic resolution. The mobile U2 small nuclear ribonucleoprotein particle (snRNP) associates with U4/U6.U5 tri-snRNP through the U2/U6 helix II and an interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which also transiently contacts the helicase Brr2. The 3' region of the U2 snRNP is flexibly attached to the SF3b-containing domain and protrudes over the concave surface of tri-snRNP, where the U1 snRNP may reside before its release from the pre-mRNA 5' splice site. The U6 ACAGAGA sequence forms a hairpin that weakly tethers the 5' splice site. The B complex proteins Prp38, Snu23 and Spp381 bind the Prp8 N-terminal domain and stabilize U6 ACAGAGA stem-pre-mRNA and Brr2-U4 small nuclear RNA interactions. These results provide important insights into the events leading to active site formation.
External linksNature / PubMed:28530653 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 17.2 Å
Structure data

EMDB-3682:
Structure of a pre-catalytic spliceosome (B1 map)
Method: EM (single particle) / Resolution: 3.9 Å

3683

5nrl

EMDB-3683: Structure of a pre-catalytic spliceosome (B2 map)
PDB-5nrl: Structure of a pre-catalytic spliceosome.
Method: EM (single particle) / Resolution: 7.2 Å

EMDB-3684:
Structure of a pre-catalytic spliceosome (B3 map)
Method: EM (single particle) / Resolution: 17.2 Å

EMDB-3685:
Structure of a pre-catalytic spliceosome (B4 map)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-3686:
Structure of a pre-catalytic spliceosome (B5 map)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-3687:
Structure of a pre-catalytic spliceosome (B6 map)
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-3688:
Structure of a pre-catalytic spliceosome (B7 map)
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsSPLICING / Spliceosome / Ribonucleoprotein

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