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Structure paper

TitleStructural Basis of RNA Polymerase I Transcription Initiation.
Journal, issue, pagesCell, Vol. 169, Issue 1, Page 120-131.e22, Year 2017
Publish dateMar 23, 2017
AuthorsChristoph Engel / Tobias Gubbey / Simon Neyer / Sarah Sainsbury / Christiane Oberthuer / Carlo Baejen / Carrie Bernecky / Patrick Cramer
External linksPubMed:28340337 / Publisher's page
MethodsEM (single particle) / X-ray diffraction
Resolution3.2 - 7.7 A
Structure data

EMDB-3590:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III)

EMDB-3591:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)

EMDB-3592:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation I)

EMDB-3593:
RNA polymerase I initially transcribing complex

EMDB-3594:
RNA polymerase I initially transcribing complex with visible tandem Winged Helix domain

PDB-5n5x:
Crystal structure of S. cerevisiae Core Factor at 3.2A resolution

PDB-5n5y:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III)

PDB-5n5z:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)

PDB-5n60:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation I)

PDB-5n61:
RNA polymerase I initially transcribing complex

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-MG:
MAGNESIUM ION

ChemComp-ZN:
ZINC ION

SourceSaccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /
Saccharomyces cerevisiae (strain atcc 204508 / s288c) / yeast /
KeywordsCryoelectron Microscopy / Crystallography, X-Ray / Models, Molecular / Multiprotein Complexes / Promoter Regions, Genetic / RNA Polymerase I / Saccharomyces cerevisiae / Saccharomyces cerevisiae Proteins / Transcription Initiation, Genetic / Transcription, Genetic / TRANSCRIPTION / Transcription / RNA polymerase I initiation / Core Factor / TRANSFERASE / Transferase / RNA polymerase I / initially transcribing complex / transferase

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