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Structure paper

TitleStructural Basis of RNA Polymerase I Transcription Initiation.
Journal, issue, pagesCell, Vol. 169, Issue 1, Page 120-131.e22, Year 2017
Publish dateMar 23, 2017
AuthorsChristoph Engel / Tobias Gubbey / Simon Neyer / Sarah Sainsbury / Christiane Oberthuer / Carlo Baejen / Carrie Bernecky / Patrick Cramer /
PubMed AbstractTranscription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron ...Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.
External linksCell / PubMed:28340337
MethodsEM (single particle) / X-ray diffraction
Resolution3.2 - 7.7 Å
Structure data

3590

5n5y

EMDB-3590, PDB-5n5y:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III)
Method: EM (single particle) / Resolution: 7.7 Å

3591

5n5z

EMDB-3591, PDB-5n5z:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)
Method: EM (single particle) / Resolution: 7.7 Å

3592

5n60

EMDB-3592, PDB-5n60:
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation I)
Method: EM (single particle) / Resolution: 7.7 Å

3593

5n61

EMDB-3593, PDB-5n61:
RNA polymerase I initially transcribing complex
Method: EM (single particle) / Resolution: 3.4 Å

3594

5n61

EMDB-3594: RNA polymerase I initially transcribing complex with visible tandem Winged Helix domain
PDB-5n61: RNA polymerase I initially transcribing complex
Method: EM (single particle) / Resolution: 6.9 Å

PDB-5o7x:
CRYSTAL STRUCTURE OF S. CEREVISIAE CORE FACTOR AT 3.2A RESOLUTION
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsTRANSCRIPTION / RNA polymerase I initiation / TRANSFERASE / RNA polymerase I / initially transcribing complex / CORE FACTOR

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