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Structure paper

TitleMediator structure and rearrangements required for holoenzyme formation.
Journal, issue, pagesNature, Vol. 544, Issue 7649, Page 196-201, Year 2017
Publish date2017--13
AuthorsKuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias
External linksPubMed:28241144 / Publisher's page
KeywordsBinding Sites / Cryoelectron Microscopy / Holoenzymes / Med14 protein, S pombe / Mediator Complex / Models, Molecular / Protein Binding / Protein Conformation / Protein Subunits / RNA Polymerase II / Schizosaccharomyces / Schizosaccharomyces pombe Proteins / Structure-Activity Relationship / TRANSCRIPTION / transcription / transcriptional / Mediator / Head / Middle / Tail / Srb / RNA polymerase II / Med / TRANSCRIPTION/TRANSFERASE / RNA / Pol2 / activation / complex / TRANSCRIPTION-TRANSFERASE complex
MethodsEM (single particle)
Resolution4.4 - 7.8 A
Structure data

Cryo-EM map of the transcriptional Mediator

Cryo-EM map of the Mediator-RNAPII complex

Cryo-EM structure of the transcriptional Mediator

Cryo-EM structure of the Mediator-RNAPII complex

SourceSchizosaccharomyces pombe / / yeast /

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Feb 20, 2018. PDBj/BINDS workshop in Osaka University

PDBj/BINDS workshop in Osaka University

    Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

    Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

    • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
    • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
    • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

    External links: The 2017 Nobel Prize in Chemistry - Press Release

    Jul 12, 2017. Major update of PDB

    Major update of PDB

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    EMN Papers

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