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| Title | Mediator structure and rearrangements required for holoenzyme formation. |
|---|---|
| Journal, issue, pages | Nature, Vol. 544, Issue 7649, Page 196-201, Year 2017 |
| Publish date | Apr 13, 2017 |
 Authors | Kuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias /  |
| PubMed Abstract | The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is ...The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals. |
 External links | Nature / PubMed:28241144 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 4.4 - 7.8 Å |
| Structure data | |
| Source |
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 Keywords |  TRANSCRIPTION / transcriptional / Mediator / Head / Middle / Tail / Srb / RNA polymerase II / Med / TRANSCRIPTION/TRANSFERASE / RNA / Pol2 / activation / complex / TRANSCRIPTION-TRANSFERASE complex |
