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Showing all 39 items for author: l. & cheng

PDB-6bgi:
Cryo-EM structure of the TMEM16A calcium-activated chloride channel in nanodisc
Method: single particle / : Dang S, Feng S, Tien J, Peters CJ, Bulkley D, Lolicato M, Zhao J, Zuberbuhler K, Ye W, Qi J, Chen T, Craik CS, Jan YN, Minor Jr DL, Cheng Y, Jan LY

PDB-6bgj:
Cryo-EM structure of the TMEM16A calcium-activated chloride channel in LMNG
Method: single particle / : Dang S, Feng S, Tien J, Peters CJ, Bulkley D, Lolicato M, Zhao J, Zuberbuhler K, Ye W, Qi L, Chen T, Craik CS, Jan YN, Minor DLJr, Cheng Y, Jan LY

PDB-6elz:
State E (TAP-Flag-Ytm1 E80A) - Visualizing the assembly pathway of nucleolar pre-60S ribosmes
Method: single particle / : Kater L, Cheng J, Barrio-Garcia C, Hurt E, Beckmann R

PDB-6em1:
State C (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes
Method: single particle / : Kater L, Cheng J, Barrio-Garcia C, Hurt E, Beckmann R

PDB-6em3:
State A architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes
Method: single particle / : Kater L, Cheng J, Barrio-Garcia C, Hurt E, Beckmann R

PDB-6em4:
State B architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes
Method: single particle / : Kater L, Cheng J, Barrio-Garcia C, Hurt E, Beckmann R

PDB-6em5:
State D architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes
Method: single particle / : Kater L, Cheng J, Barrio-Garcia C, Hurt E, Beckmann R

PDB-5xs4:
Structure of Coxsackievirus A6 (CVA6) virus A-particle
Method: single particle / : Zheng QB, He MZ, Xu LF, Yu H, Li SW, Cheng T

PDB-5xs5:
Structure of Coxsackievirus A6 (CVA6) virus procapsid particle
Method: single particle / : Zheng QB, He MZ, Xu LF, Yu H, Cheng T, Li SW

PDB-5xs7:
Structure of Coxsackievirus A6 (CVA6) virus A-particle in complex with the neutralizing antibody fragment 1D5
Method: single particle / : Zheng QB, He MZ, Xu LF, Yu H, Li SW, Cheng T

PDB-5v93:
Cryo-EM structure of the 70S ribosome from Mycobacterium tuberculosis bound with Capreomycin
Method: single particle / : Yang K, Chang JY, Cui Z, Li X, Meng R, Duan L, Thongchol J, Jakana J, Huwe C, Sacchettini J, Zhang J

PDB-5vkq:
Structure of a mechanotransduction ion channel Drosophila NOMPC in nanodisc
Method: single particle / : Jin P, Bulkley D, Guo Y, Zhang W, Guo Z, Huynh W, Wu S, Meltzer S, Chen T, Jan LY, Jan YN, Cheng Y

PDB-5wsn:
Structure of Japanese encephalitis virus
Method: single particle / : Wang X, Zhu L, Li S, Yuan S, Qin C, Fry EE, Stuart ID, Rao Z

PDB-5h0r:
RNA dependent RNA polymerase ,vp4,dsRNA
Method: single particle / : Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L

PDB-5h0s:
EM Structure of VP1A and VP1B
Method: single particle / : Li X, Zhou N, Xu B, Chen W, Zhu B, Wang X, Wang J, Liu H, Cheng L

PDB-5h4p:
Structural snapshot of cytoplasmic pre-60S ribosomal particles bound with Nmd3, Lsg1, Tif6 and Reh1
Method: single particle / : Ma C, Wu S, Li N, Chen Y, Yan K, Li Z, Zheng L, Lei J, Woolford JL, Gao N

PDB-5wua:
Structure of a Pancreatic ATP-sensitive Potassium Channel
Method: single particle / : Li N, Wu JX, Chen L, Gao N

PDB-3jct:
Cryo-em structure of eukaryotic pre-60S ribosomal subunits
Method: single particle / : Wu S, Kumcuoglu B, Yan KG, Brown H, Zhang YX, Tan D, Gamalinda M, Yuan Y, Li ZF, Jakovljevic J, Ma CY, Lei JL, Dong MQ, Woolford Jr JL, Gao N

PDB-3ja4:
RNA-dependent RNA polymerases of transcribing cypoviruses
Method: single particle / : Liu H, Cheng L

PDB-3ja5:
Genome and RdRp structure within the capsid of no-transcribing cypovirus
Method: single particle / : Liu H, Cheng L

PDB-3j9b:
Electron cryo-microscopy of an RNA polymerase
Method: single particle / : Chang SH, Sun DP, Liang HH, Wang J, Li J, Guo L, Wang XL, Guan CC, Boruah BM, Yuan LM, Feng F, Yang MR, Wojdyla J, Wang JW, Wang MT, Wang HW, Liu YF

PDB-3j6q:
Identification of the active sites in the methyltransferases of a transcribing dsRNA virus
Method: single particle / : Zhu B, Yang C, Liu H, Cheng L, Song F, Zeng S, Huang X, Ji G, Zhu P

PDB-3j7l:
Full virus map of brome mosaic virus
Method: single particle / : Wang Z, Hryc C, Bammes B, Afonine PV, Jakana J, Chen DH, Liu X, Baker ML, Kao C, Ludtke SJ, Schmid MF, Adams PD, Chiu W

PDB-3j7m:
Virus model of brome mosaic virus (first half data set)
Method: single particle / : Wang Z, Hryc C, Bammes B, Afonine PV, Jakana J, Chen DH, Liu X, Baker ML, Kao C, Ludtke SJ, Schmid MF, Adams PD, Chiu W

PDB-3j7n:
Virus model of brome mosaic virus (second half data set)
Method: single particle / : Wang Z, Hryc C, Bammes B, Afonine PV, Jakana J, Chen DH, Liu X, Baker ML, Kao C, Ludtke SJ, Schmid MF, Adams PD, Chiu W

PDB-3j6r:
Electron cryo-microscopy of Human Papillomavirus Type 16 capsid
Method: single particle / : Cardone G, Moyer AL, Cheng N, Thompson CD, Dvoretzky I, Lowy DR, Schiller JT, Steven AC, Buck CB, Trus BL

PDB-3zif:
Cryo-EM structures of two intermediates provide insight into adenovirus assembly and disassembly
Method: single particle / : Cheng L, Huang X, Li X, Xiong W, Sun W, Yang C, Zhang K, Wang Y, Liu H, Ji G, Sun F, Zheng C, Zhu P

PDB-4btq:
Coordinates of the bacteriophage phi6 capsid subunits fitted into the cryoEM map EMD-1206
Method: single particle / : Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC

PDB-4btg:
Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution
Method: single particle / : Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC

PDB-3j17:
Structure of a transcribing cypovirus by cryo-electron microscopy
Method: single particle / : Yang C, Ji G, Liu H, Zhang K, Liu G, Sun F, Zhu P, Cheng L

PDB-3iz3:
CryoEM structure of cytoplasmic polyhedrosis virus
Method: single particle / : Cheng L, Sun J, Zhang K, Mou Z, Huang X, Ji G, Sun F, Zhang J, Zhu P

PDB-3iys:
Homology model of avian polyomavirus asymmetric unit
Method: single particle / : Shen PS, Enderlein D, Nelson CDS, Carter WS, Kawano M, Xing L, Swenson RD, Olson NH, Baker TS, Cheng RH, Atwood WJ, Johne R, Belnap DM

PDB-3iyo:
Cryo-EM model of virion-sized HEV virion-sized capsid
Method: single particle / : Xing L, Mayazaki N, Li TC, Simons MN, Wall JS, Moore M, Wang CY, Takeda N, Wakita T, Miyamura T, Cheng RH

PDB-3k1q:
Backbone model of an aquareovirus virion by cryo-electron microscopy and bioinformatics
Method: single particle / : Cheng LP, Zhu J, Hiu WH, Zhang XK, Honig B, Fang Q, Zhou ZH

PDB-3c91:
Thermoplasma acidophilum 20S proteasome with an open gate
Method: single particle / : Rabl J, Smith DM, Yu Y, Chang SC, Goldberg AL, Cheng Y

PDB-3c92:
Thermoplasma acidophilum 20S proteasome with a closed gate
Method: single particle / : Rabl J, Smith DM, Yu Y, Chang SC, Goldberg AL, Cheng Y

PDB-2o0f:
Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3
Method: single particle / : Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H

PDB-2fte:
Bacteriophage HK97 Expansion Intermediate IV
Method: single particle / : Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE

PDB-1if0:
PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)
Method: single particle / : Conway JF, Wikoff WR, Cheng N, Duda RL, Hendrix RW, Johnson JE, Steven AC

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Oct 4, 2017. Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

Three pioneers of this field were awarded Nobel Prize in Chemistry 2017

  • Jacques Dubochet (University of Lausanne, Switzerland) is a pioneer of ice-embedding method of EM specimen (as known as cryo-EM), Most of 3DEM structures in EMDB and PDB are obtained using his method.
  • Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images).
  • Richard Henderson (MRC Laboratory of Molecular Biology, Cambridge, UK) was determined the first biomolecule structure by EM. The first EM entry in PDB, PDB-1brd is determinedby him.

External links: The 2017 Nobel Prize in Chemistry - Press Release

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