+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-8622 | |||||||||||||||
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Title | S. cerevisiae U1 snRNP | |||||||||||||||
Map data | S. cerevisiae U1 snRNP | |||||||||||||||
Sample |
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Function / homology | Function and homology information positive regulation of RNA binding / splicing factor binding / mRNA splice site recognition / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / spliceosomal tri-snRNP complex / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / positive regulation of mRNA splicing, via spliceosome ...positive regulation of RNA binding / splicing factor binding / mRNA splice site recognition / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / spliceosomal tri-snRNP complex / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / positive regulation of mRNA splicing, via spliceosome / U2-type prespliceosome assembly / commitment complex / U4 snRNP / U2 snRNP / poly(U) RNA binding / U1 snRNP / U2-type prespliceosome / pre-mRNA 5'-splice site binding / precatalytic spliceosome / spliceosomal complex assembly / mRNA 5'-splice site recognition / U5 snRNP / spliceosomal snRNP assembly / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / spliceosomal complex / mRNA splicing, via spliceosome / mRNA binding / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) / Baker's yeast (brewer's yeast) / Saccharomyces cerevisiae S288c (yeast) | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.7 Å | |||||||||||||||
Authors | Li X / Liu S / Jiang J / Zhang L / Espinosa S / Hill RC / Hansen KC / Zhou ZH / Zhao R | |||||||||||||||
Funding support | United States, 4 items
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Citation | Journal: Nat Commun / Year: 2017 Title: CryoEM structure of Saccharomyces cerevisiae U1 snRNP offers insight into alternative splicing. Authors: Xueni Li / Shiheng Liu / Jiansen Jiang / Lingdi Zhang / Sara Espinosa / Ryan C Hill / Kirk C Hansen / Z Hong Zhou / Rui Zhao / Abstract: U1 snRNP plays a critical role in 5'-splice site recognition and is a frequent target of alternative splicing factors. These factors transiently associate with human U1 snRNP and are not amenable for ...U1 snRNP plays a critical role in 5'-splice site recognition and is a frequent target of alternative splicing factors. These factors transiently associate with human U1 snRNP and are not amenable for structural studies, while their Saccharomyces cerevisiae (yeast) homologs are stable components of U1 snRNP. Here, we report the cryoEM structure of yeast U1 snRNP at 3.6 Å resolution with atomic models for ten core proteins, nearly all essential domains of its RNA, and five stably associated auxiliary proteins. The foot-shaped yeast U1 snRNP contains a core in the "ball-and-toes" region architecturally similar to the human U1 snRNP. All auxiliary proteins are in the "arch-and-heel" region and connected to the core through the Prp42/Prp39 paralogs. Our demonstration that homodimeric human PrpF39 directly interacts with U1C-CTD, mirroring yeast Prp42/Prp39, supports yeast U1 snRNP as a model for understanding how transiently associated auxiliary proteins recruit human U1 snRNP in alternative splicing. | |||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_8622.map.gz | 116.1 MB | EMDB map data format | |
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Header (meta data) | emd-8622-v30.xml emd-8622.xml | 28.7 KB 28.7 KB | Display Display | EMDB header |
Images | emd_8622.png | 57 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-8622 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-8622 | HTTPS FTP |
-Related structure data
Related structure data | 6n7xMC 5uz5 M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_8622.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | S. cerevisiae U1 snRNP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.36 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
+Entire : S. cerevisiae U1 snRNP
+Supramolecule #1: S. cerevisiae U1 snRNP
+Macromolecule #1: U1 small nuclear ribonucleoprotein 70 kDa homolog
+Macromolecule #2: U1 small nuclear ribonucleoprotein C
+Macromolecule #3: U1 small nuclear ribonucleoprotein A,TAP tag
+Macromolecule #4: U1 small nuclear ribonucleoprotein component PRP42
+Macromolecule #5: Pre-mRNA-processing factor 39
+Macromolecule #6: Protein NAM8
+Macromolecule #7: 56 kDa U1 small nuclear ribonucleoprotein component
+Macromolecule #8: Protein LUC7
+Macromolecule #9: Small nuclear ribonucleoprotein-associated protein B
+Macromolecule #10: Small nuclear ribonucleoprotein Sm D1
+Macromolecule #11: Small nuclear ribonucleoprotein Sm D2
+Macromolecule #12: Small nuclear ribonucleoprotein Sm D3
+Macromolecule #13: Small nuclear ribonucleoprotein E
+Macromolecule #14: Small nuclear ribonucleoprotein F
+Macromolecule #15: Small nuclear ribonucleoprotein G
+Macromolecule #16: RNA
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 1.1 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
CTF correction | Software - Name: CTFFIND (ver. 4) |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0) |
Final 3D classification | Software - Name: RELION (ver. 2.0) |
Final angle assignment | Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.0) |
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2) / Number images used: 352900 |
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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Output model | PDB-6n7x: |