[English] 日本語
Yorodumi
- EMDB-8402: Methicillin sensitive Staphylococcus aureus 70S ribosome -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-8402
TitleMethicillin sensitive Staphylococcus aureus 70S ribosome
Map dataMethicillin sensitive Staphylococcus aureus 70S ribosome
Sample
  • Complex: Methicillin sensitive Staphylococcus aureus 70S ribosome (ATCC 35556)
    • RNA: x 6 types
    • Protein or peptide: x 46 types
  • Ligand: x 2 types
Function / homology
Function and homology information


ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit ...ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / transferase activity / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / zinc ion binding / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 ...Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein S15, bacterial-type / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein S6 / Ribosomal protein S6
Similarity search - Domain/homology
50S ribosomal protein L32 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 ...50S ribosomal protein L32 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein bS20 / 50S ribosomal protein L33 1 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS15 / 30S ribosomal protein S10
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria) / Staphylococcus aureus (strain NCTC 8325) (bacteria) / Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsEyal Z / Ahmed T / Belousoff N / Mishra S / Matzov D / Bashan A / Zimmerman E / Lithgow T / Bhushan S / Yonath A
Funding support Australia, European Union, 2 items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)1092262 Australia
European Research Council (ERC)322581 (NOVRIB)European Union
CitationJournal: mBio / Year: 2017
Title: Structural Basis for Linezolid Binding Site Rearrangement in the Ribosome.
Authors: Matthew J Belousoff / Zohar Eyal / Mazdak Radjainia / Tofayel Ahmed / Rebecca S Bamert / Donna Matzov / Anat Bashan / Ella Zimmerman / Satabdi Mishra / David Cameron / Hans Elmlund / Anton Y ...Authors: Matthew J Belousoff / Zohar Eyal / Mazdak Radjainia / Tofayel Ahmed / Rebecca S Bamert / Donna Matzov / Anat Bashan / Ella Zimmerman / Satabdi Mishra / David Cameron / Hans Elmlund / Anton Y Peleg / Shashi Bhushan / Trevor Lithgow / Ada Yonath /
Abstract: An unorthodox, surprising mechanism of resistance to the antibiotic linezolid was revealed by cryo-electron microscopy (cryo-EM) in the 70S ribosomes from a clinical isolate of This high-resolution ...An unorthodox, surprising mechanism of resistance to the antibiotic linezolid was revealed by cryo-electron microscopy (cryo-EM) in the 70S ribosomes from a clinical isolate of This high-resolution structural information demonstrated that a single amino acid deletion in ribosomal protein uL3 confers linezolid resistance despite being located 24 Å away from the linezolid binding pocket in the peptidyl-transferase center. The mutation induces a cascade of allosteric structural rearrangements of the rRNA that ultimately results in the alteration of the antibiotic binding site. The growing burden on human health caused by various antibiotic resistance mutations now includes prevalent resistance to last-line antimicrobial drugs such as linezolid and daptomycin. Structure-informed drug modification represents a frontier with respect to designing advanced clinical therapies, but success in this strategy requires rapid, facile means to shed light on the structural basis for drug resistance (D. Brown, Nat Rev Drug Discov 14:821-832, 2015, https://doi.org/10.1038/nrd4675). Here, detailed structural information demonstrates that a common mechanism is at play in linezolid resistance and provides a step toward the redesign of oxazolidinone antibiotics, a strategy that could thwart known mechanisms of linezolid resistance.
History
DepositionSep 15, 2016-
Header (metadata) releaseDec 14, 2016-
Map releaseMay 24, 2017-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: RCSB / Status: Released

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-5tcu
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8402.png

Downloads & links

-
Map

FileDownload / File: emd_8402.map.gz / Format: CCP4 / Size: 262.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMethicillin sensitive Staphylococcus aureus 70S ribosome
Voxel sizeX=Y=Z: 0.96 Å
Density
Contour LevelBy AUTHOR: 0.065 / Movie #1: 0.065
Minimum - Maximum-0.20492297 - 0.37027705
Average (Standard dev.)-0.00033267733 (±0.021430288)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions410410410
Spacing410410410
CellA=B=C: 393.6 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.960.960.96
M x/y/z410410410
origin x/y/z0.0000.0000.000
length x/y/z393.600393.600393.600
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS410410410
D min/max/mean-0.2050.370-0.000

-
Supplemental data

-
Sample components

+
Entire : Methicillin sensitive Staphylococcus aureus 70S ribosome (ATCC 35556)

EntireName: Methicillin sensitive Staphylococcus aureus 70S ribosome (ATCC 35556)
Components
  • Complex: Methicillin sensitive Staphylococcus aureus 70S ribosome (ATCC 35556)
    • RNA: 16S RRNA
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: mRNAMessenger RNA
    • RNA: P-site tRNA CHAIN
    • RNA: E-site tRNA CHAIN
    • RNA: 23S RRNA23S ribosomal RNA
    • RNA: 5S rRNA CHAIN
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 1Ribosome
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31 type BRibosome
  • Ligand: PAROMOMYCIN
  • Ligand: MAGNESIUM ION

+
Supramolecule #1: Methicillin sensitive Staphylococcus aureus 70S ribosome (ATCC 35556)

SupramoleculeName: Methicillin sensitive Staphylococcus aureus 70S ribosome (ATCC 35556)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52
Source (natural)Organism: Staphylococcus aureus (bacteria)

+
Macromolecule #1: 16S RRNA

MacromoleculeName: 16S RRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 502.935844 KDa
SequenceString: UUUAUGGAGA GUUUGAUCCU GGCUCAGGAU GAACGCUGGC GGCGUGCCUA AUACAUGCAA GUCGAGCGAA CGGACGAGAA GCUUGCUUC UCUGAUGUUA GCGGCGGACG GGUGAGUAAC ACGUGGAUAA CCUACCUAUA AGACUGGGAU AACUUCGGGA A ACCGUAGC ...String:
UUUAUGGAGA GUUUGAUCCU GGCUCAGGAU GAACGCUGGC GGCGUGCCUA AUACAUGCAA GUCGAGCGAA CGGACGAGAA GCUUGCUUC UCUGAUGUUA GCGGCGGACG GGUGAGUAAC ACGUGGAUAA CCUACCUAUA AGACUGGGAU AACUUCGGGA A ACCGUAGC UAAUACCGGA UAAUAUUUUG AACCGCAUGG UUCAAAAGUG AAAGACGGUC UUGCUGUCAC UUAUAGAUGG AU CCGCGCU GCAUUAGCUA GUUGGUAAGG UAACGGCUUA CCAAGGCAAC GAUGCAUAGC CGACCUGAGA GGGUGAUCGG CCA CACUGG AACUGAGACA CGGUCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGCAAUGGGC GAAAGCCUGA CGGA GCAAC GCCGCGUGAG UGAUGAAGGU CUUCGGAUCG UAAAACUCUG UUAUUAGGGA AGAACAUAUG UGUAAGUAAC UGUGC ACAU CUUGACGGUA CCUAAUCAGA AAGCCACGGC UAACUACGUG CCAGCAGCCG CGGUAAUACG UAGGUGGCAA GCGUUA UCC GGAAUUAUUG GGCGUAAAGC GCGCGUAGGC GGUUUUUUAA GUCUGAUGUG AAAGCCCACG GCUCAACCGU GGAGGGU CA UUGGAAACUG GAAAACUUGA GUGCAGAAGA GGAAAGUGGA AUUCCAUGUG UAGCGGUGAA AUGCGCAGAG AUAUGGAG G AACACCAGUG GCGAAGGCGA CUUUCUGGUC UGUAACUGAC GCUGAUGUGC GAAAGCGUGG GGAUCAAACA GGAUUAGAU ACCCUGGUAG UCCACGCCGU AAACGAUGAG UGCUAAGUGU UAGGGGGUUU CCCGCCCCUU AGUGCUGCAG CUAACGCAUU AAGCACUCC GCCUGGGGAG UACGACCGCA AGGUUGAAAC UCAAAGGAAU UGACGGGGAC CCGCACAAGC GGUGGAGCAU G UGGUUUAA UUCGAAGCAA CGCGAAGAAC CUUACCAAAU CUUGACAUCC UUUGACAACU CUAGAGAUAG AGCCUUCCCC UU CGGGGGA CAAAGUGACA GGUGGUGCAU GGUUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGC AACCCU UAAGCUUAGU UGCCAUCAUU AAGUUGGGCA CUCUAAGUUG ACUGCCGGUG ACAAACCGGA GGAAGGUGGG GAUG ACGUC AAAUCAUCAU GCCCCUUAUG AUUUGGGCUA CACACGUGCU ACAAUGGACA AUACAAAGGG CAGCGAAACC GCGAG GUCA AGCAAAUCCC AUAAAGUUGU UCUCAGUUCG GAUUGUAGUC UGCAACUCGA CUACAUGAAG CUGGAAUCGC UAGUAA UCG UAGAUCAGCA UGCUACGGUG AAUACGUUCC CGGGUCUUGU ACACACCGCC CGUCACACCA CGAGAGUUUG UAACACC CG AAGCCGGUGG AGUAACCUUU UAGGAGCUAG CCGUCGAAGG UGGGACAAAU GAUUGGGGUG AAGUCGUAAC AAGGUAGC C GUAUCGGAAG GUGCGGCUGG AUCACCUCCU UUCU

+
Macromolecule #20: mRNA

MacromoleculeName: mRNA / type: rna / ID: 20 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria)
Molecular weightTheoretical: 4.157573 KDa
SequenceString:
AAAAAUGUUC AAA

+
Macromolecule #21: P-site tRNA CHAIN

MacromoleculeName: P-site tRNA CHAIN / type: rna / ID: 21 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria)
Molecular weightTheoretical: 24.156334 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACC

+
Macromolecule #22: E-site tRNA CHAIN

MacromoleculeName: E-site tRNA CHAIN / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus subsp. aureus NCTC 8325 (bacteria)
Molecular weightTheoretical: 24.485539 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

+
Macromolecule #23: 23S RRNA

MacromoleculeName: 23S RRNA / type: rna / ID: 23 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 946.687688 KDa
SequenceString: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCAAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA ...String:
GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCAAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA GCAUAUCAGA AGGCACACCC GGAGAACUGA AACAUCUUAG UACCCGGAGG AAGAGAAAGA AAAUUCGAUU CC CUUAGUA GCGGCGAGCG AAACGGGAAG AGCCCAAACC AACAAGCUUG CUUGUUGGGG UUGUAGGACA CUCUAUACGG AGU UACAAA GGACGACAUU AGACGAAUCA UCUGGAAAGA UGAAUCAAAG AAGGUAAUAA UCCUGUAGUC GAAAAUGUUG UCUC UCUUG AGUGGAUCCU GAGUACGACG GAGCACGUGA AAUUCCGUCG GAAUCUGGGA GGACCAUCUC CUAAGGCUAA AUACU CUCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAUAGAAC CUGAAA CCG UGUGCUUACA AGUAGUCAGA GCCCGUUAAU GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUUUG AU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCUUGG UCAGGUUGAA GUUCAGGUAA CACUGAAUGG AGGACCGAAC CGACUUACGU UGAAAAGUG AGCGGAUGAA CUGAGGGUAG CGGAGAAAUU CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGGGCUA GCCUCAAGU GAUGAUUAUU GGAGGUAGAG CACUGUUUGG ACGAGGGGCC CCUCUCGGGU UACCGAAUUC AGACAAACUC C GAAUGCCA AUUAAUUUAA CUUGGGAGUC AGAACAUGGG UGAUAAGGUC CGUGUUCGAA AGGGAAACAG CCCAGACCAC CA GCUAAGG UCCCAAAAUA UAUGUUAAGU GGAAAAGGAU GUGGCGUUGC CCAGACAACU AGGAUGUUGG CUUAGAAGCA GCC AUCAUU UAAAGAGUGC GUAAUAGCUC ACUAGUCGAG UGACACUGCG CCGAAAAUGU ACCGGGGCUA AACAUAUUAC CGAA GCUGU GGAUUGUCCU UUGGACAAUG GUAGGAGAGC GUUCUAAGGG CGUUGAAGCA UGAUCGUAAG GACAUGUGGA GCGCU UAGA AGUGAGAAUG CCGGUGUGAG UAGCGAAAGA CGGGUGAGAA UCCCGUCCAC CGAUUGACUA AGGUUUCCAG AGGAAG GCU CGUCCGCUCU GGGUUAGUCG GGUCCUAAGC UGAGGCCGAC AGGCGUAGGC GAUGGAUAAC AGGUUGAUAU UCCUGUA CC ACCUAUAAUC GUUUUAAUCG AUGGGGGGAC GCAGUAGGAU AGGCGAAGCG UGCGAUUGGA UUGCACGUCU AAGCAGUA A GGCUGAGUAU UAGGCAAAUC CGGUACUCGU UAAGGCUGAG CUGUGAUGGG GAGAAGACAU UGAGUCUUCG AGUCGUUGA UUUCACACUG CCGAGAAAAG CCUCUAGAUA GAAAAUAGGU GCCCGUACCG CAAACCGACA CAGGUAGUCA AGAUGAGAAU UCUAAGGUG AGCGAGCGAA CUCUCGUUAA GGAACUCGGC AAAAUGACCC CGUAACUUCG GGAGAAGGGG UGCUCUUUAG G GUUAACGC CCAGAAGAGC CGCAGUGAAU AGGCCCAAGC GACUGUUUAU CAAAAACACA GGUCUCUGCU AAACCGUAAG GU GAUGUAU AGGGGCUGAC GCCUGCCCGG UGCUGGAAGG UUAAGAGGAG UGGUUAGCUU CUGCGAAGCU ACGAAUCGAA GCC CCAGUA AACGGCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAAU UCCUUGUCGG GUAAGUUCCG ACCCGCACGA AAGG CGUAA CGAUUUGGGC ACUGUCUCAA CGAGAGACUC GGUGAAAUCA UAGUACCUGU GAAGAUGCAG GUUACCCGCG ACAGG ACGG AAAGACCCCG UGGAGCUUUA CUGUAGCCUG AUAUUGAAAU UCGGCACAGC UUGUACAGGA UAGGUAGGAG CCUUUG AAA CGUGAGCGCU AGCUUACGUG GAGGCGCUGG UGGGAUACUA CCCUAGCUGU GUUGGCUUUC UAACCCGCAC CACUUAU CG UGGUGGGAGA CAGUGUCAAG CGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCUC AAAGGUUC C CUCAGAAUGG UUGGAAAUCA UUCAUAGAGU GUAAAGGCAU AAGGGAGCUU GACUGCGAGA CCUACAAGUC GAGCAGGGU CGAAAGACGG ACUUAGUGAU CCGGUGGUUC CGCAUGGAAG GGCCAUCGCU CAACGGAUAA AAGCUACCCC GGGGAUAACA GGCUUAUCU CCCCCAAGAG UUCACAUCGA CGGGGAGGUU UGGCACCUCG AUGUCGGCUC AUCGCAUCCU GGGGCUGUAG U CGGUCCCA AGGGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGCGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCC CU AUCCGUC GUGGGCGUAG GAAAUUUGAG AGGAGCUGUC CUUAGUACGA GAGGACCGGG AUGGACAUAC CUCUGGUGUA CCA GUUGUC GUGCCAACGG CAUAGCUGGG UAGCUAUGUG UGGACGGGAU AAGUGCUGAA AGCAUCUAAG CAUGAAGCCC CCCU CAAGA UGAGAUUUCC CAACUUCGGU UAUAAGAUCC CUCAAAGAUG AUGAGGUUAA UAGGUUCGAG GUGGAAGCAU GGUGA CAUG UGGAGCUGAC GAAUACUAAU CGAUCGAAGA CUUAAUCAA

+
Macromolecule #24: 5S rRNA CHAIN

MacromoleculeName: 5S rRNA CHAIN / type: rna / ID: 24 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 36.692801 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGGUC UUUAGCGACG AUGGUAGCCA ACUUACGUU CCGCUAGAGU AGAACGUUGC CAGGC

+
Macromolecule #2: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 22.684201 KDa
SequenceString: GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE ...String:
GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE GTVPLHTLRA DIDYAHAEAD TTYGKLGVKV WIYR

+
Macromolecule #3: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 22.849145 KDa
SequenceString: RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD ...String:
RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD SLTGTFVRLP ERSELPAEIN EQLIVEYYSR

+
Macromolecule #4: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 16.522119 KDa
SequenceString:
EFEERVVTIN RVAKVVKGGR RFRFTALVVV GDKNGRVGFG TGKAQEVPEA IKKAVEAAKK DLVVVPRVEG TTPHTITGRY GSGSVFMKP AAPGTGVIAG GPVRAVLELA GITDILSKSL GSNTPINMVR ATIDGLQNLK NAEDVAKLRG KTVEELY

+
Macromolecule #5: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 11.239766 KDa
SequenceString:
MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIRED

+
Macromolecule #6: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 17.695357 KDa
SequenceString:
PRKGSVPKRD VLPDPIHNSK LVTKLINKIM LDGKRGTAQR ILYSAFDLVE QRSGRDALEV FEEAINNIMP VLEVKARRVG GSNYQVPVE VRPERRTTLG LRWLVNYARL RGEKTMEDRL ANEILDAANN TGGAVKKRED THKMAEANKA FAHYRW

+
Macromolecule #7: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 14.622014 KDa
SequenceString:
MTDPIADMLT RVRNANMVRH EKLELPASNI KKEIAEILKS EGFIKNVEYV EDDKQGVLRL FLKYGQNDER VITGLKRISK PGLRVYAKA SEMPKVLNGL GIALVSTSEG VITDKEARKR NVGGEIIAYV W

+
Macromolecule #8: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 14.312321 KDa
SequenceString:
VEYRGTGRRK NSVARVRLVP GEGNITVNNR DVREYLPFES LILDLNQPFD VTETKGNYDV LVNVHGGGFT GQAQAIRHGI ARALLEADP EYRGSLKRAG LLTRDPRMKE RKKPGLKAAR RSPQFSKR

+
Macromolecule #9: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 9.24361 KDa
SequenceString:
IRIRLKAYDH RVIDQSAEKI VETAKRSGAD VSGPIPLPTE KSVYTIIRAV HKYKDSREQF EQRTHKRLID IVNPTPKTVD

+
Macromolecule #10: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 12.047609 KDa
SequenceString:
NIENGVAHIR STFNNTIVTI TDEFGNALSW SSAGALGFKG SKKSTPFAAQ MASETASKSA MEHGLKTVEV TVKGPGPGRE SAIRALQSA GLEVTAIRDV TPVPHNGCRP PKRRR

+
Macromolecule #11: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 15.189673 KDa
SequenceString:
PTINQLVRKP RQSKIKKSDS PALNKGFNSK KKKFTDLNSP QKRGVCTRVG TMTPKKPNSA LRKYARVRLS NNIEINAYIP GIGHNLQEH SVVLVRGGRV KDLPGVRYHI VRGALDTSGV DGRRQGRSLY GTKKPKN

+
Macromolecule #12: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 12.843768 KDa
SequenceString:
ARIAGVDIPR EKRVVISLTY IYGIGTSTAQ KILEEANVSA DTRVKDLTDD ELGRIREVVD GYKVEGDLRR ETNLNIKRLM EISSYRGIR HRRGLPVRGQ KTKNNARTRK GPVK

+
Macromolecule #13: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 7.186573 KDa
SequenceString:
AKTSMVAKQQ KKQKYAVREY TRCERCGRPH SVYRKFKLCR ICFRELAYKG QIPGVRKASW

+
Macromolecule #14: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 10.503135 KDa
SequenceString:
AISQERKNEI IKEYRVHETD TGSPEVQIAV LTAEINAVNE HLRTHKKDHH SRRGLLKMVG RRRHLLNYLR SKDIQRYREL IKSLGIRR

+
Macromolecule #15: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 9.291743 KDa
SequenceString:
AVKIRLTRLG SKRNPFYRIV VADARSPRDG RIIEQIGTYN PTSANAPEIK VDEALALKWL NDGAKPTDTV HNILSKEGIM KKF

+
Macromolecule #16: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 9.36686 KDa
SequenceString:
NDRKVYVGKV VSDKMDKTIT VLVETYKTHK LYGKRVKYSK KYKTHDENNS AKLGDIVKIQ ETRPLSATKR FRLVEIVEES

+
Macromolecule #17: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 6.574833 KDa
SequenceString:
GITHIDYKDT ELLKRFISER GKILPRRVTG TSAKYQRMLT TAIKRSRHMA LLPYVK

+
Macromolecule #18: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 9.010406 KDa
SequenceString:
SIKKGPFVDE HLMKKVEAQE GSEKKQVIKT WSRRSTIFPN FIGHTFAVYD GRKHVPVYVT EDMVGHKLGE FAPTRTFK

+
Macromolecule #19: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 8.609938 KDa
SequenceString:
KSAIKRVKTT EKAEARNISQ KSAMRTAVKN AKTAVSNNAD NKNELVSLAV KLVDKAAQSN LIHSNKADRI KSQLMTANK

+
Macromolecule #25: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 29.827607 KDa
SequenceString: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG ...String:
AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG KYVLIRLRSG EVRMILSTCR ATIGQVGNLQ HELVNVGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRPSPMS PWGKPTLGKK TRRGKKSSDK LIVRGRK

+
Macromolecule #26: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 23.199545 KDa
SequenceString: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM ...String:
TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM PGRMGGNTVT VQNLEVVQVD TENKVILVKG NVPGPKKGLV EIRTSIK

+
Macromolecule #27: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 22.293424 KDa
SequenceString: NYDVLKLDGT KSGSIELSDA VFGIEPNNSV LFEAINLQRA SLRQGTHAVK NRSAVSGGGR KPWKQKGTGR ARQGTIRAPQ WRGGGIVFG PTPRSYAYKM PKKMRRLALR SALSFKAQEN GLTVVDAFNF EAPKTKEFKN VLSTLEQPKK VLVVTENEDV N VELSARNI ...String:
NYDVLKLDGT KSGSIELSDA VFGIEPNNSV LFEAINLQRA SLRQGTHAVK NRSAVSGGGR KPWKQKGTGR ARQGTIRAPQ WRGGGIVFG PTPRSYAYKM PKKMRRLALR SALSFKAQEN GLTVVDAFNF EAPKTKEFKN VLSTLEQPKK VLVVTENEDV N VELSARNI PGVQVTTAQG LNVLDITNAD SLVITEAAAK KVEEVLG

+
Macromolecule #28: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 18.758744 KDa
SequenceString:
NRLKEKFNTE VTENLMKKFN YSSVMEVPKI DKIVVNMGVG DAVQNSKVLD NAVEELELIT GQKPLVTKAK KSIATFRLRE GMPIGAKVT LRGERMYEFL DKLISVSLPR VRDFQGVSKK AFDGRGNYTL GVKEQLIFPE IDYDKVSKVR GMDIVIVTTA N TDEEAR

+
Macromolecule #29: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 19.400051 KDa
SequenceString:
SRVGKKIIDI PSDVTVTFDG NHVTVKGPKG ELSRTLNERM TFKQEENTIE VVRPSDSKED RTNHGTTRAL LNNMVQGVSQ GYVKVLELV GVGYRAQMQG KDLILNVGYS HPVEIKAEEN ITFSVEKNTV VKVEGISKEQ VGALASNIRS VRPPEPYKGK G IRYQGEYV RRKEGK

+
Macromolecule #30: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 16.359427 KDa
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

+
Macromolecule #31: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 13.157342 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

+
Macromolecule #32: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 15.515731 KDa
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEV

+
Macromolecule #33: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 15.486276 KDa
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREE

+
Macromolecule #34: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 13.42035 KDa
SequenceString:
RKLGRTSDQR KAMLRDLATS LIISERIETT EARAKEVRSV VEKLITLGKK GDLASRRNAA KTLRNVEILN EDETTQTALQ KLFGEIAER YTERQGGYTR ILKQGPRRGD GAESVIIELV

+
Macromolecule #35: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 12.461404 KDa
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAAR

+
Macromolecule #36: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 12.46363 KDa
SequenceString:
TNHKLIEAVT KSQLRTDLPS FRPGDTLRVH VRIIEGTRER IQVFEGVVIK RRGGGVSETF TVRKISSGVG VERTFPLHTP KIEKIEVKR RGKVRRAKLY YLRSLRGKAA

+
Macromolecule #37: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 13.459919 KDa
SequenceString:
PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL

+
Macromolecule #38: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 11.354081 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

+
Macromolecule #39: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 12.328358 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKE

+
Macromolecule #40: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 10.3801 KDa
SequenceString:
EARDILKRPV ITEKSSEAMA EDKYTFDVDT RVNKTQVKMA VEEIFNVKVA SVNIMNYKPK KKRMGRYQGY TNKRRKAIVT LKEGSIDLF

+
Macromolecule #41: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 11.316205 KDa
SequenceString:
HIKKGDNVKV IAGKDKGKEG KVIATLPKKD RVVVEGVNIM KKHQKPTQLN PEGGILETEA AIHVSNVQLL DPKTNEPTRV GYKFVDGKK VRIAKKSGEE IKSN

+
Macromolecule #42: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 10.391122 KDa
SequenceString:
SLKSIIRQGK QTRSDLKQLR KSGKVPAVVY GYGTKNVSVK VDEVEFIKVI REVGRNGVIE LGVGSKTIKV MVADYQFDPL KNQITHIDF LAIN

+
Macromolecule #43: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 8.911099 KDa
SequenceString:
KKGVSSTKNG RDSESKRLGA KRADGQFVTG GSILYRQRGT KIYPGENVGR GGDDTLFAKI DGVVKFERKG RDKKQVSVYA VA

+
Macromolecule #44: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 6.550716 KDa
SequenceString:
KQCFVTGRKA STGNRRSHAL NSTKRRWNAN LQKVRILVDG KPKKVWVSAR ALKSGKVT

+
Macromolecule #45: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 7.331325 KDa
SequenceString:
EIRDLTTSEI EEQIKSSKEE LFNLRFQLAT GQLEETARIR TVRKTIARLK TVAREREIEQ SK

+
Macromolecule #46: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 6.305308 KDa
SequenceString:
AKLQITLTRS VIGRPETQRK TVEALGLKKT NSSVVVEDNP AIRGQINKVK HLVTVEE

+
Macromolecule #47: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 5.406392 KDa
SequenceString:
AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGEYKLSHR VCKNCGS

+
Macromolecule #48: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 5.684561 KDa
SequenceString:
RVNVTLACTE CGDRNYITTK NKRNNPERIE MKKYCPRLNK YTLHRET

+
Macromolecule #49: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 5.252369 KDa
SequenceString:
VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLS

+
Macromolecule #50: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 6.985402 KDa
SequenceString:
PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL

+
Macromolecule #51: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

+
Macromolecule #52: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (strain NCTC 8325) (bacteria)
Strain: NCTC 8325
Molecular weightTheoretical: 8.603602 KDa
SequenceString:
MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGR VERF

+
Macromolecule #53: PAROMOMYCIN

MacromoleculeName: PAROMOMYCIN / type: ligand / ID: 53 / Number of copies: 1 / Formula: PAR
Molecular weightTheoretical: 615.628 Da
Chemical component information

ChemComp-PAR:
PAROMOMYCIN / Antimicrobial, medication*YM / Paromomycin

+
Macromolecule #54: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 129 / Formula: MG
Molecular weightTheoretical: 24.305 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration0.4 mg/mL
BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 20.0 nm
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TECNAI ARCTICA
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3j9z

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 126000

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-5tcu:
Methicillin sensitive Staphylococcus aureus 70S ribosome

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more