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- EMDB-6784: Large subunit of Trichomonas vaginalis ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-6784
TitleLarge subunit of Trichomonas vaginalis ribosome
Map data
Sample
  • Complex: Large subunit of Trichomonas vaginalis ribosome
    • RNA: x 3 types
    • Protein or peptide: x 40 types
Function / homology
Function and homology information


protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / assembly of large subunit precursor of preribosome / modification-dependent protein catabolic process / protein tag activity / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / ribosome biogenesis ...protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / assembly of large subunit precursor of preribosome / modification-dependent protein catabolic process / protein tag activity / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / ribosome biogenesis / 5S rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / translation / mRNA binding / ubiquitin protein ligase binding / RNA binding / metal ion binding / nucleus / cytoplasm
Similarity search - Function
: / : / : / Ribosomal protein L29e / Ribosomal protein L10e / Ribosomal L29e protein family / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily ...: / : / : / Ribosomal protein L29e / Ribosomal protein L10e / Ribosomal L29e protein family / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal L27e protein family / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L34e / Ribosomal protein L37ae / 60S ribosomal protein L6E / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L7A/L8 / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal L37ae protein family / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L14e domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / 60S ribosomal protein L19 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L37e / Ribosomal protein L14 / Ribosomal protein L21e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L14 / Ribosomal protein L31e / Ribosomal protein L37ae/L37e / Ribosomal protein L14, KOW motif / Ribosomal protein L24e-related / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L39e / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L39e domain superfamily / Ribosomal_L19e / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L18e / Ribosomal L39 protein / Ribosomal protein L37e / Ribosomal protein L24e / Ribosomal L15 / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L7/L30 / Ribosomal proteins L26 eukaryotic, L24P archaeal / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L32e / Ribosomal protein L32e superfamily / Ribosomal_L32e / Ribosomal protein L32 / Ribosomal protein L1e signature. / Ribosomal protein L21e signature. / Ribosomal protein L37e signature.
Similarity search - Domain/homology
60S ribosomal protein L18, putative / 60S ribosomal protein L19-3, putative / 60S ribosomal protein L39, putative / Ribosomal protein L31e, putative / 60S ribosomal protein L44, putative / Uncharacterized protein / Ribosomal protein L6, putative / Uncharacterized protein / Ubiquitin / Ribosomal_L18e/L15P domain-containing protein ...60S ribosomal protein L18, putative / 60S ribosomal protein L19-3, putative / 60S ribosomal protein L39, putative / Ribosomal protein L31e, putative / 60S ribosomal protein L44, putative / Uncharacterized protein / Ribosomal protein L6, putative / Uncharacterized protein / Ubiquitin / Ribosomal_L18e/L15P domain-containing protein / Ribosomal protein L24e, putative / Ribosomal protein L24, putative / 60S ribosomal protein L29 / Ribosomal protein L22, putative / Ribosomal protein L27e, putative / Ribosomal protein L5 / 60S ribosomal protein L22-1, putative / 60S ribosomal protein L23, putative / Ribosomal protein L35Ae, putative / Ribosomal protein L23, putative / Ribosomal protein L13, putative / Ribosomal protein L14, putative / Ribosomal protein, putative / Ribosomal protein L29, putative / Ribosomal protein L8, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L11, putative / Ribosomal protein L15 / 60S ribosomal protein L18a / 60S ribosomal protein L7a / Ribosomal protein L36e, putative / Ribosomal protein L32, putative / Ribosomal protein L21e, putative / Ribosomal protein L30p/L7e, putative / Ribosomal protein L37 / Ribosomal protein L38e, putative / Ribosomal protein, putative / Ribosomal protein L34e, putative / 60S ribosomal protein L6, putative / Ribosomal protein L13e, putative
Similarity search - Component
Biological speciesTrichomonas vaginalis (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsLi Z / Guo Q / Zheng L / Ji Y / Xie Y / Lai D / Lun Z / Suo X / Gao N
CitationJournal: Cell Res / Year: 2017
Title: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.
Authors: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao /
Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.
History
DepositionJul 6, 2017-
Header (metadata) releaseAug 30, 2017-
Map releaseAug 30, 2017-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.044
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.044
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5xy3
  • Surface level: 0.044
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_6784.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.32 Å
Density
Contour LevelBy AUTHOR: 0.044 / Movie #1: 0.044
Minimum - Maximum-0.23113365 - 0.39924592
Average (Standard dev.)0.0004943846 (±0.01008034)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 422.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2310.3990.000

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Supplemental data

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Mask #1

Fileemd_6784_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Large subunit of Trichomonas vaginalis ribosome

EntireName: Large subunit of Trichomonas vaginalis ribosome
Components
  • Complex: Large subunit of Trichomonas vaginalis ribosome
    • RNA: 25S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: Ribosomal protein L8, putativeRibosome
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Ribosomal protein, putativeRibosome
    • Protein or peptide: Ribosomal protein L560S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6, putativeRibosome
    • Protein or peptide: Ribosomal protein L30p/L7e, putativeRibosome
    • Protein or peptide: Ribosomal protein L7Ae, putativeRibosome
    • Protein or peptide: Ribosomal protein L6, putativeRibosome
    • Protein or peptide: Ribosomal protein, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L11, putativeRibosome
    • Protein or peptide: Ribosomal protein L13e, putativeRibosome
    • Protein or peptide: Ribosomal protein L14, putativeRibosome
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Ribosomal protein L13, putativeRibosome
    • Protein or peptide: Ribosomal protein L22, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L18, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L19-3, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: Ribosomal protein L21e, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L22-1, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L23, putativeRibosome
    • Protein or peptide: Ribosomal protein L24e, putativeRibosome
    • Protein or peptide: Ribosomal protein L23, putativeRibosome
    • Protein or peptide: Ribosomal protein L24, putativeRibosome
    • Protein or peptide: Ribosomal protein L27e, putativeRibosome
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30, putativeRibosome
    • Protein or peptide: Ribosomal protein L31e, putativeRibosome
    • Protein or peptide: Ribosomal protein L32, putativeRibosome
    • Protein or peptide: Ribosomal protein L35Ae, putativeRibosome
    • Protein or peptide: Ribosomal protein L34e, putativeRibosome
    • Protein or peptide: Ribosomal protein L29, putativeRibosome
    • Protein or peptide: Ribosomal protein L36e, putativeRibosome
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: Ribosomal protein L38e, putativeRibosome
    • Protein or peptide: 60S ribosomal protein L39, putativeRibosome
    • Protein or peptide: Ubiquitin, putative
    • Protein or peptide: 60S ribosomal protein L44, putativeRibosome
    • Protein or peptide: Uncharacterized protein

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Supramolecule #1: Large subunit of Trichomonas vaginalis ribosome

SupramoleculeName: Large subunit of Trichomonas vaginalis ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Trichomonas vaginalis (eukaryote)

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Macromolecule #1: 25S ribosomal RNA

MacromoleculeName: 25S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 892.223312 KDa
SequenceString: CGUAGUCUGC CAUACGCAGG AAGACCCGCU GAACUGAAGC AUCUCAUUAA GCGGAGGAAA AGAAACUAAC UAGGAUUCCC UUAGUAAAG GCGAUCGAAA AGGGAACAGC CCAACGUCUU AAUCCCGCAA GGGAAAUGUA GUCGGUUCAC CUAUUGCACU G GUCUAAGU ...String:
CGUAGUCUGC CAUACGCAGG AAGACCCGCU GAACUGAAGC AUCUCAUUAA GCGGAGGAAA AGAAACUAAC UAGGAUUCCC UUAGUAAAG GCGAUCGAAA AGGGAACAGC CCAACGUCUU AAUCCCGCAA GGGAAAUGUA GUCGGUUCAC CUAUUGCACU G GUCUAAGU CAGAUGGAAU GCUGCGCGAU UCAGAGUAAA AGCCUCGUAU GACCAACCGC AAGCUUACGA UGGACCGGGG AG AGUCGGA UACUUUGAGA GUAGUGUCUU AAGGGGGUGG UAUGUCCCAU CCAAGGCUAA AUAACGUGAG GAGACCGAUA GAU UACAAG UAGUGUGAAC GAAAGAUGAA AAGAACUCUA AAACAGAGGG UGAAAAGUUC CUGAACUUGC UUAGGGUUAA GGCU AUUUA UAGUACCCGU CUUGAAACAC GGAUUAGGGA GUUUAUCUGU AGUGCGAGUC UUUGCACGUU AACUGUAAAG GCGUA GAGA AAGCAACUGG CGUUUGCAUG GCUACUGAGC AUUACUGAUA GGACCCGAAA GGUGGUGAAC UUUGCCUGCA CAGGUU GAA GUCAGGGGAA ACUCUGAUGG AGGACCGUAC GGGUUAUUGA UGUGCAAAUC AUUUUUCUGA UGUAGGUAAA GGUGCGA AA GACUCAUCGA ACCACCUAAU AGCUGGUUUC CUCCGAAGUU UCCCUCAGGA UAGCUGUAAU GCAUUUAGUU AAGGGGAG U AGAGAUAUGG GUAGAGGUGC AGGGAGCCUU CGGGUUCUCA ACUUAUUUCC AAACUUCGAA CCUCCUUGUU AGGCAUUUA CCUAACUGGA AAUGCUUAAA UGAAUUAGCG UUACAAGUGG GCUCUGACCG GUAAGCGGUC AUGGCGAAAU GGGAUGAACC AUACGUCUG AAUAAGGUGC CGAAAUGUAC UAUCACUGAU ACCAUAAAGG GAGUCCAUGU AUUUAGACAG CAGGUCGGUG C CCCUAAAA GCAGGUAUCC GCUAAAGAGU GUGUUAAAAC UCACCUGCCG AAUACUAGGG CCCCGAAAAU GAAUGGCGCA UA UAGUACU ACCGAUCUCA GACAUCAGUU CUAUUGAACU GAGAGUAGGG GGAUGUGGAC AUUUUUGUGA AGGUGCAAUG UGA GUUGGA CUGGAAGAAU GUCUAUUACA GAUCCUAAUU GAAGUAGCGA UUAUCCAGGC UUGUAUCCUG GGGGCUGAUG AGGA GUAGG GUUUCUGGAC AACUUAAACA GAUCCAGAGU UAGUCGAUCC UAAUCUCAAU AAUGGGAGAA AGGGAGUACA GUUGA UAUU CUGUAACUGA CAACAGUUCG CGGCAACGCA UACUAUUUUU CUCCGCCAAU CUUAUACCGU UGGUAGGGAU CUCUUU CCU ACAUGACAGU ACUAAUACCC AGGGAAACUG GUCAUCAGGC GAACUGCGGC GUUCUGCUGG CAAAACAUAG CUUCGGU UA UGUCAAUGGC UAUUGGAUUG GUCCAUGAAG CGAAAAAUAG UUUACUGUGU CACUCGUACC GAUAACCGCA UCAGGUCU U CUAGGUAAGU AGCCUAUGGC CAUUGAGGCA UGAUAGUUCA GGGAAUUCGG CCAAUUAGAG CUGUAACUUC GGGAUAAAG CUUGGCUCUG AUGAAAAUCA GAACCGAUUG UUACGCGGAG AGCCCAACUG UUUAAUAAAA ACGUAGUGUC GCGCUGUGGU AGUAUAGCC AGUGAACGCG ACAUGAAUCC UGCUCAGUGC UGUGUCUGUU AAUAUGGUUC AAGCCAACGA AGCACCGGUA A AUAGCUGG GGUAACUAUG ACUCUUAUCA AAGUAGCGAA AUACCUCGUC ACUUAAUUAG UGACCUGCAU GAACGGAGCU AU GAGGUUC UCACUGUCCC AAACUGUCUC UCAGCGAACC AACUGUAGUG GGAACGGUCA CUAGAUAUCC AGCGGGGAAA GAA GACCCU GUAGAGCUUC ACUUUGGUCU AGUCUUGUAU AGUUCCUGCG GGGGUGUAGA CUAGGUGGGA GAGCAAUCGA UUCU GAAAA ACCACUACCC UGUUUGGAAU UAUGCUUACC UAUCACAAGG AAUAGGACUA GAUAGAAAGU UUGGCUGGGG CGGCA CACC UUCGAAAGAG CAGCGAAGGU GUCCUAAGUU GAGCUCAGUG AGGACAGAAA UCUCACGUUG AGGAUAAGGC CAAAAG CUC AAUUUACUGU GCCUUUUUGU ACUAGGAUCA GUUACGAAAG UAAGGCCUAA CGAUCCCUAA AUAGCCUCAG GUGCGUG AG GAUUGGGGUG ACAGACAAGU UACCUCCGGG ACAACUGGCU UGUGGCUGCU AAGAGAUCAA AUCAACGCAG CUUUUUGA U UCUUCGAUGU CGGCUCUUCC UAUCCUAGUG AUGUACAAGU UGCUAAGGGU CGGAUUGUUC ACCCGUAUUA GGGAUCGUG AGCUGGGUUU AGACCGUCGU GAGCCAGGUU UGUUUUAUCC UACUGGAUAU AGCAUCAUGA UAGUAGAUUG AUUUAGUACG AGAGGAACU GUUGAUUAUG GCCUCUGGUU AGCGAUUAUC GGAAACGGUA UGUCGCGCAG CUACGCCGUC UGGGAUAAUG G CUGAAAGC CUCUUAAGCC AGAAGCCCAU CUAGAAAGUG AUGCACGUGA UGUUGGACCG AUACGAAUAA UCAUACUCAA AA GGUCCGC UUCAUAGGUA ACCCAAUGUA GAAGACAUUG UGUGAACGAA GGUACUGUAA GCAGUGGAGU GGCAUUACGU CCA CGACCU GUUGAGGUUC AGCUUCGCUU CUAGGUUUCC AAAUAUUUU

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Macromolecule #2: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 37.963594 KDa
SequenceString:
GAAGCGGCCA CACCCGGCUG GCAACAUCCA GCUCCACUCC GAACCUGGAA AUUAAGCAGU CGCGGGCCAC AUCAGUACUG GGCUGGGCG ACUUCCCGGG AACGUGCGGU GCUUGCUUC

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Macromolecule #3: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 52.173945 KDa
SequenceString:
AAGUCUCUAA GCAAUGGAUG UCUUGGCUCC UCACACGAUG AAGAACGUGG CAUAAUGUGU UAAGUAACCG GAGUUGCAAA CAUCAUGAC AGGUUAAUCU UUGAAUGCAA AUUGCGCUAA ACUCGAUCUC GGUCGAGAAG CAUGGGUGUG ACAGUACUAC A UC

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Macromolecule #4: Ribosomal protein L8, putative

MacromoleculeName: Ribosomal protein L8, putative / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 26.949082 KDa
SequenceString: MGHIVRAQRR GNGSVFQAHT HHRVGPAKFR ALDAAERTSV ISGMVKEIIH DPGRGAPLAK LIYKKAEGQG FDSALVIAPE GIHTGQFIK CGAQADLHIG NILPLAQIPE GTEICNVEHR PGDGGRYGRC SGDSCRVIGH TENYTRIQLP SGRKALVSNI C RATLGIVA ...String:
MGHIVRAQRR GNGSVFQAHT HHRVGPAKFR ALDAAERTSV ISGMVKEIIH DPGRGAPLAK LIYKKAEGQG FDSALVIAPE GIHTGQFIK CGAQADLHIG NILPLAQIPE GTEICNVEHR PGDGGRYGRC SGDSCRVIGH TENYTRIQLP SGRKALVSNI C RATLGIVA GGGRPEKPLL KAGNVHYKYK AKRHTWPVVC GIKMNPVDHR HGGGSHQHMG APGTVARSAR PGQKLGLIAS RR TGRRRGT VNLA

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Macromolecule #5: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 46.90427 KDa
SequenceString: MSHRKFNAPR RGSLGFLPRG RSHAVRGRVR SWPKDDASQK PHLCAFIGYK AGMTHVLRDV VRPNSRLHKK EACEPVTILE TPPMFVVGI IGYKPTVEGL KPVTTVWASY VNEEVKRNYY KNWYQSKARK AFSCLSNGKA AEKREKQLEE LQKEATVIRV I AHTQSAKT ...String:
MSHRKFNAPR RGSLGFLPRG RSHAVRGRVR SWPKDDASQK PHLCAFIGYK AGMTHVLRDV VRPNSRLHKK EACEPVTILE TPPMFVVGI IGYKPTVEGL KPVTTVWASY VNEEVKRNYY KNWYQSKARK AFSCLSNGKA AEKREKQLEE LQKEATVIRV I AHTQSAKT TTRGVDANEQ GAKKVLKGNH LGQKKAHMIE IQINGGDVAA KLNYAKSILE KEIKVADVFT EGEQIDTIGV GK GFGWEGV IHRYGTKRLQ KKTHRGRRKV ACIGPWNPAR VLWSVARYGQ RGCHHRTEMN KRIYRIGAAE VEGKINEGGS TSF DLTKKS INPMGGFPHY GLVKDDFLMI KGSVVGTVKR AITLRKTINI NTRRIATEEI NLKWIDTASK FGHGRFQTKE ERSK FLGKL KISKKNETQQ

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Macromolecule #6: Ribosomal protein, putative

MacromoleculeName: Ribosomal protein, putative / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 40.320738 KDa
SequenceString: MSRQTVNVLA QDGQKASTIE LPKVFDTPIR AEVVKEVYVN LAKNAQQPHA NDPMAGKKVS AISWGTGRAK ACVPRVNGSG SNRNGQGAY ANFCRGGHRF NPPTLLRRWF RPVPSRQRKF AIASAIAATA VVPLVQARGH VLGEVKEVPI VVVDAVQEIK R TRDAVELL ...String:
MSRQTVNVLA QDGQKASTIE LPKVFDTPIR AEVVKEVYVN LAKNAQQPHA NDPMAGKKVS AISWGTGRAK ACVPRVNGSG SNRNGQGAY ANFCRGGHRF NPPTLLRRWF RPVPSRQRKF AIASAIAATA VVPLVQARGH VLGEVKEVPI VVVDAVQEIK R TRDAVELL KKVGVYGDVQ RVLDGSVHRS SKGKFRRAAY KTKKGPLVIY NEDKGIVKAF RNIPGVETIS VKALALAKLA PA AQVGRLT VWTESAFKAL DGIYESKKRF SLPRSIMTNA DIEAVITSDA VQSVLNEKKE VVPLPKCRCC KKLSVGACED WQK ALKEVA ELRAAQEAKR TSPEVVKAVF AEAVAAQPAT PDNMSTQIIN HIPL

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Macromolecule #7: Ribosomal protein L5

MacromoleculeName: Ribosomal protein L5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 34.932766 KDa
SequenceString: MSAKLVKNAG YFSRFQTKFR RRREAKTDYV QRTQLIQQDK TKYGAAKYRL VARITNTKVI AQIVVAELTG DKTVCQALST ELPKYGIKL GLSNYPAAYA TGLLVARRFL TQMKLADVFK TEITEEYLND EENRRPFKVI LDVGLARTTT GAKVFAVMKG A VDGGLFIP ...String:
MSAKLVKNAG YFSRFQTKFR RRREAKTDYV QRTQLIQQDK TKYGAAKYRL VARITNTKVI AQIVVAELTG DKTVCQALST ELPKYGIKL GLSNYPAAYA TGLLVARRFL TQMKLADVFK TEITEEYLND EENRRPFKVI LDVGLARTTT GAKVFAVMKG A VDGGLFIP HNVCRLACKF DKKDKDAGKD RKDVPADKIV KGNQYYILGG AVADYMRKLK KESEEKYNKQ FSRYVKAGIT AD NLEKIYK DAHAAIRKNP AATVIADKKK HAEEMKQKHA PKKPQTKKLS FEEKRKILNE HLVAAGLPPR K

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Macromolecule #8: 60S ribosomal protein L6, putative

MacromoleculeName: 60S ribosomal protein L6, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 16.045772 KDa
SequenceString:
MAELLFISPI AKKDIKRPSW RGIPRISFTR PAVAAEVDPI AKPACNAKAV ETRANLKVGT VVIIVGGEHQ GKRAVVVADQ GAGIVKVAG PVVPVNEISQ DYLIATSTSI DVAANATEAQ VEAAAAKVPE MVDYLKAPFT IKKGNRIHLM KF

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Macromolecule #9: Ribosomal protein L30p/L7e, putative

MacromoleculeName: Ribosomal protein L30p/L7e, putative / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 26.842281 KDa
SequenceString: MGKPLPETLQ KIAARVEAQK AAYLKKKEEL LAAAKANEAK IQERAKKYAA EYVSQTKAEI DAENKATAEG AFYVPAEAKF AFVIRTKGT NKLHPDVRKI LHLFRLNQKH NAIFVRLNKA TIEMLKRVTP QVAFGYPSVD MVRKLIYKLG TANLNGQRIP I ADNQIIKV ...String:
MGKPLPETLQ KIAARVEAQK AAYLKKKEEL LAAAKANEAK IQERAKKYAA EYVSQTKAEI DAENKATAEG AFYVPAEAKF AFVIRTKGT NKLHPDVRKI LHLFRLNQKH NAIFVRLNKA TIEMLKRVTP QVAFGYPSVD MVRKLIYKLG TANLNGQRIP I ADNQIIKV ALGHLCIESV EDLAHEIYTV GPNFAAANRF LAVFKLHAPK GGYKKINRAY VEGGDYGNRE HLIDELIERM I

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Macromolecule #10: Ribosomal protein L7Ae, putative

MacromoleculeName: Ribosomal protein L7Ae, putative / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 27.602273 KDa
SequenceString: MAPKATRSAP EKVIEDKDII RQEHLFAEET AEKVADKTTQ TRFPKYVQLQ RQKRILMKRL KVPPPVNHFN HTLGKDAAVA LFKFLEKYR PETKTEKKQR NKDDAEKAKK DLKVAGSGNK KALVQGVKNV TAAIESKKAQ LVIIAHDVDP IELVIWMPAL C RNLEIPYC ...String:
MAPKATRSAP EKVIEDKDII RQEHLFAEET AEKVADKTTQ TRFPKYVQLQ RQKRILMKRL KVPPPVNHFN HTLGKDAAVA LFKFLEKYR PETKTEKKQR NKDDAEKAKK DLKVAGSGNK KALVQGVKNV TAAIESKKAQ LVIIAHDVDP IELVIWMPAL C RNLEIPYC IVKSKSRLGQ IVGMKTCSCV ALAEVKPEDR AAFTKIVDSV NSGFLAHYKE EMHQWGGGEL SEKTIEKLKA QG KYKE

+
Macromolecule #11: Ribosomal protein L6, putative

MacromoleculeName: Ribosomal protein L6, putative / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 22.930506 KDa
SequenceString: MPRPVLHQEH FKIPENIKCT VKSGVVTIEH QDPKDKKTTK LVKDLSHLKL DFEYDEKDHQ IVARCWFGNR KLLARIGTLF GIVKNMITG VTLGWRYKMH FVYSHFPIKH TCSEDGRTFD FNGFMGHKEH KIVTAPEGVR IWSNESVAKD EINIEGANLE D VSLVCGQI ...String:
MPRPVLHQEH FKIPENIKCT VKSGVVTIEH QDPKDKKTTK LVKDLSHLKL DFEYDEKDHQ IVARCWFGNR KLLARIGTLF GIVKNMITG VTLGWRYKMH FVYSHFPIKH TCSEDGRTFD FNGFMGHKEH KIVTAPEGVR IWSNESVAKD EINIEGANLE D VSLVCGQI HQLTKIKDKD LRKFLDGIYV QHIEHLKEE

+
Macromolecule #12: Ribosomal protein, putative

MacromoleculeName: Ribosomal protein, putative / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 23.800781 KDa
SequenceString: MGRRPARCYR YIGNKPYPKS RFCRGVPDPR IIRYETGTKT AHAKELPICC RLILLEHECV SSEALEAARV SSNRYMMKTM GKDGYHIKI NLHPFHVLRI NKMLSTAGAD RLQTGMRHAF GKPEGLVARA NYNSQIMTIR TTSAGLKHAL EALRRASFKF P GHSRVVIS ...String:
MGRRPARCYR YIGNKPYPKS RFCRGVPDPR IIRYETGTKT AHAKELPICC RLILLEHECV SSEALEAARV SSNRYMMKTM GKDGYHIKI NLHPFHVLRI NKMLSTAGAD RLQTGMRHAF GKPEGLVARA NYNSQIMTIR TTSAGLKHAL EALRRASFKF P GHSRVVIS NKVGFTPYTF EQFKELQANG QIVDCGNHVK RVYRRGPIPT H

+
Macromolecule #13: 60S ribosomal protein L11, putative

MacromoleculeName: 60S ribosomal protein L11, putative / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 19.373488 KDa
SequenceString:
MSNKLNEIRV AKLCININPG AVGDQVTRAS RVIEQLTKQQ AMLARSHRTI PKFGIRRNDK IAAYATIRGP KALEILNRAL QVHDYEISG RCFSIEGNFG FGIKEHIDLG LKYDRDIGIH GMDLYVVLER PGNRISRRKH CQSRIGPNQR VKREEAKEWF V QKFNGTLL K

+
Macromolecule #14: Ribosomal protein L13e, putative

MacromoleculeName: Ribosomal protein L13e, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 21.865705 KDa
SequenceString:
MVAKNNQIPN DHLRKYWYHR VKTFFDDPAR AQRRRNARNL RAKKIAPRPA EGPLRPIVRC PTVRYNMKTR LGRGFTPKEL VAAGFDPAL ARFQGIAVDA RRAHSKDAMV KQNVERLQAY KARLIKVKKG ETVEQVNDAA LFALPKAQNE IKFRVIKAEE A KKDLFKEK TDKLKAFREA KKEKAAAKHK K

+
Macromolecule #15: Ribosomal protein L14, putative

MacromoleculeName: Ribosomal protein L14, putative / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 14.726112 KDa
SequenceString:
MPFKQFVEIG RVALINFGEL VDKLAIIVDI VDDKRVLIDV LEFDAPRQTI PIQRLKLTDY VAKIQRNAAP EAAKAAAKDF IKQFYESAW GKQVIARKNK AQMNDFQRFK YAQLEAKRDA LVAKELNNH

+
Macromolecule #16: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 24.323465 KDa
SequenceString: MGAFTYLREL YTKKQSEVMR FILRLRCWEY RQLPELVRLT HPSRPDKARR MGYKAKQGMV VYRIRVRKGS RRRVASKNRQ YGKPRTHHI YKLTPSLNLQ AIAEQKAGKQ LPSLRLLNSY WVNQDGAFKY FECIFVDPVH PAIQHDAKLN WICDSVHTRR E ARGLTAAG ...String:
MGAFTYLREL YTKKQSEVMR FILRLRCWEY RQLPELVRLT HPSRPDKARR MGYKAKQGMV VYRIRVRKGS RRRVASKNRQ YGKPRTHHI YKLTPSLNLQ AIAEQKAGKQ LPSLRLLNSY WVNQDGAFKY FECIFVDPVH PAIQHDAKLN WICDSVHTRR E ARGLTAAG RKHRGLSGVG HGFRHLRPSR YADWKVRNVF KLRRWR

+
Macromolecule #17: Ribosomal protein L13, putative

MacromoleculeName: Ribosomal protein L13, putative / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 22.569111 KDa
SequenceString: MSAPIVIDAK GHIQGRLAAA VAKQLLKGRK VVVVRCEEIE LNGQHKFTVH TYERFLNKTT NSNPRDGPFH QRAPHEIFAR AVRGMIPYK THRGADAFAN LQCYEGIPAK YVSYKRLVIP HALRCVSIHT DRPTTKLGRL STTMGWKYGN VVAQLEEQRK A ESAKYFEA ...String:
MSAPIVIDAK GHIQGRLAAA VAKQLLKGRK VVVVRCEEIE LNGQHKFTVH TYERFLNKTT NSNPRDGPFH QRAPHEIFAR AVRGMIPYK THRGADAFAN LQCYEGIPAK YVSYKRLVIP HALRCVSIHT DRPTTKLGRL STTMGWKYGN VVAQLEEQRK A ESAKYFEA KKAAQDKRAA AIEKVNKQLG AQAVKFLETF QE

+
Macromolecule #18: Ribosomal protein L22, putative

MacromoleculeName: Ribosomal protein L22, putative / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 18.53049 KDa
SequenceString:
MSTHYTFSNV EESKALKAST GLERVHFKPM METGRVIKGM KAQDAIAYLE AVMRKERAVP FFRYKGGISH HAQGHEWGCP TSRWPVKCA ELYIKLIKTA LAGAPQKPGV NVDEVLVAHV QCNRATQYRW RRIHQAHGRV KSYASPPTNV QIVLAEKAAI K ERSQ

+
Macromolecule #19: 60S ribosomal protein L18, putative

MacromoleculeName: 60S ribosomal protein L18, putative / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 21.122545 KDa
SequenceString:
MGVDLDHRVP RKQRQEPKTA DAQMKMLHNL YAFLARRTGN DFNAKIAHRL CISRTNRRPY SLSRLATALN GKEAETIAVL VAKITNDER LLEVPKMKVC ALKFTETARA RILAAGGECI TFDQLAQLRP TGDKCLLLEG DRTRRLVNKH FGAAPGDDNS K TRPKVASH GRKFEMARGR RKSRWYHA

+
Macromolecule #20: 60S ribosomal protein L19-3, putative

MacromoleculeName: 60S ribosomal protein L19-3, putative / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 21.056785 KDa
SequenceString:
MSSLKLQKRL AASVLDCGVR RVWMDDSEVS TIAMANSRQQ IRKLINDGII VKRPVKVHSG ARHTKRILAK RLGRHTGYGK RRGGRNARL TGKFQWIRRI RVLRKVLVDY RKRNEIDCHL YRKLYLKAKG NEFRNRRNLV EHIQTLKAEN LREKTLQSQI D ARRARSRR VRERKNGVSQ

+
Macromolecule #21: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 19.853973 KDa
SequenceString:
MSLKQYLVIG RPYPTKEIPN PPAVRVQVFA PDEVIAKSKF WTVAQRIARL KKAHGEVLAV KHVIEPEPTR VKNYGVWLSY RSTRNHHNV YKEYRDTTTE GAATKMLAEL AGTHDVSADN ISIIKIAEVA DKDLVHKNIS QFTHENIKFP MLKQSIRAPT A SARKVFAA TRPTVCGF

+
Macromolecule #22: Ribosomal protein L21e, putative

MacromoleculeName: Ribosomal protein L21e, putative / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 17.910867 KDa
SequenceString:
MGHTEGLRHA TRYLFARKFR KHGAPAPSIT LRTFRIGDIV DIKVNGALHS GMPNRVYHGA TGIVWNVSPH AVGILVKRRV NNHIAPKRI YVRTEHVFPS KSKNDFVARC QANLKAHEEA KKAGTKIALP KRLPAQPRAS HVVKPELIQT ITDAPFRENW

+
Macromolecule #23: 60S ribosomal protein L22-1, putative

MacromoleculeName: 60S ribosomal protein L22-1, putative / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 12.237054 KDa
SequenceString:
MASSVFYVDC SAPVADKFFQ LQEFVEYLQS HMKVDNLRKN LANKVTIEAD AGANKIVVNA SVKYSKRAVR YYARKFLAKK DIRSRFHVI ASGKDTYELR PYRVSNE

+
Macromolecule #24: 60S ribosomal protein L23, putative

MacromoleculeName: 60S ribosomal protein L23, putative / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 14.840554 KDa
SequenceString:
MSKRGRTGQQ GTKFAMTAGL PVGAVINCCD NSGAKNMFII SVRGHKGRLN RLPAASVSDL IVVTCKKGKP ALRKKVSMGV VVRQRAIWR RKDGVVIGFQ DNAGVIINDK GEMKGSAITG PVAKEAAELW PKVASVAPAV F

+
Macromolecule #25: Ribosomal protein L24e, putative

MacromoleculeName: Ribosomal protein L24e, putative / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 13.282475 KDa
SequenceString:
MSTKTEICGF SGHIFHAGHG RVHIREDKHL MAFESRKAFR MWERKRNPRK IAWTEHYRHD HKKSVADTAA TEKRAQRKKA TRTYAGVAM EGNQHQMVQI VKKRVARKPV AKKN

+
Macromolecule #26: Ribosomal protein L23, putative

MacromoleculeName: Ribosomal protein L23, putative / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 15.582249 KDa
SequenceString:
MSGKAQAAAQ TLAQGQKKEV TKTRNFTKFG KNAAQVVPRR PAFEHKAVKP VSLKHNDYKI IRGPVTSDKS TVKIENENTM TFWVDIKAT KTEIAAAFNR LYKVKPVKVC TVITSKFVKK AYIRLPANVE AMNIATEIGF A

+
Macromolecule #27: Ribosomal protein L24, putative

MacromoleculeName: Ribosomal protein L24, putative / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 15.782617 KDa
SequenceString:
MVKTNPFVSS QARKVRKSYF NAKKDAKHVA MSAPLSKELQ EANGIKRLPI RRDDEVMIFS GRMQNRTGKV TAVKLSEMRI YVDTFTTEK INGQAVSFPV HPSNVVITKL KMDKARKNLI ELRRLGRDQI LAKLGHKKQ

+
Macromolecule #28: Ribosomal protein L27e, putative

MacromoleculeName: Ribosomal protein L27e, putative / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 15.946061 KDa
SequenceString:
MSLLQAGRFV VMLQGRHAGK KAVVLAAYPE GTEDRKFPYA IVMGIEKYPK KVTKDMPTEV LVKKTQVKLF VKAVNFNHVM LTRHNVKDE DFFKKISVEK VVAALKDAPA KKALLTEYAA VLRQKYLNNK MAWFFKPLQF

+
Macromolecule #29: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 17.006787 KDa
SequenceString:
MPTRFHKSRK LRGQVSMGFG RVGKHRVSPG GHGRAGGQHH QRTWFDRFHP GYFGKVGMRH LHLMKNRADE YEPTINVDKL WTLVPEEVR VKAAAAKKGD AVPVIDCVQN GFFKVLARGE LPKIPFVVRA KDFSEEAEKK IKAAGGVCEK VQ

+
Macromolecule #30: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 6.823724 KDa
SequenceString:
MAKRSNTSAH NRSHQHHRNG IHKCVVKKLW FEKGMNAKFL RNARYAKAGQ NKSEEEDE

+
Macromolecule #31: 60S ribosomal protein L30, putative

MacromoleculeName: 60S ribosomal protein L30, putative / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 12.316229 KDa
SequenceString:
MGRKKLSRAR ESIINSLSLV TKSGKYQLGI DKTLQSLRNG EAKLVIFASN VAPAHRSLIE YYAMLSGCDI LPFDGDNVDL GTACGKYFR SSVISIIDAG ESEILKMIKQ KDE

+
Macromolecule #32: Ribosomal protein L31e, putative

MacromoleculeName: Ribosomal protein L31e, putative / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 12.446528 KDa
SequenceString:
MPKELAPAKI ETTIHLQKAV HGTTRRFRAE RAIKVIAAAA KKMMHTDVVK IDPELNSRIW IHGKNCPPTR VRVTFERKAD EEDAEKMIT IASFKNVASF NGLQTTKVVD Q

+
Macromolecule #33: Ribosomal protein L32, putative

MacromoleculeName: Ribosomal protein L32, putative / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 15.249669 KDa
SequenceString:
MVNPAEHKPI IHKKTNRFHR PQSDLWNRVP SAWRHPRGID SRFRRKYRGT PLHPSAGFGS DKETRFMLPN GFIPVIVRSL KDLDMLLTK NTTHGAMISA QVGAKLQQEI IKKAQELNIE ILNQARKHVA QEQ

+
Macromolecule #34: Ribosomal protein L35Ae, putative

MacromoleculeName: Ribosomal protein L35Ae, putative / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 11.668678 KDa
SequenceString:
MSEQVKLFVP ATFVSFRRSK RIIKPNQALL EIQGVVSKEE AQFYAGKKVA SVWKFDKQEF INWGVITQPH GNSGVVRAKF EKNLPPQML GKQVRVMLYP STI

+
Macromolecule #35: Ribosomal protein L34e, putative

MacromoleculeName: Ribosomal protein L34e, putative / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 13.346646 KDa
SequenceString:
MVDNRYTLRR SLGFHTIRNR VAPVRTPGNK LVAHYIAKRQ NGPHCAETGK RINGIKCVKT CELRRMKKNQ RTVSRPYGGV YCGSVVKDR IINAFMEEEA RAAQERKMAN EQKKKGK

+
Macromolecule #36: Ribosomal protein L29, putative

MacromoleculeName: Ribosomal protein L29, putative / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 14.300049 KDa
SequenceString:
MGHLKGKELI KKTRQELLDE LAGLEKKLFE LKGQKAASAA ATKLTEIKNT RRDVARVKSI LMVSQRKALQ EKYAKAKLVP IDLRKHQEK SLRNKLAPKY ANKLTAQESR RHKCLFERKF ALKAQ

+
Macromolecule #37: Ribosomal protein L36e, putative

MacromoleculeName: Ribosomal protein L36e, putative / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 10.941717 KDa
SequenceString:
MSEEQQKVLA PKRECGLHSS GKRVNFARKI VHELVGWQPY ERHTMDLIKI ERRRAARQFL KKRLGTHRRA MKKLNTLEEV VQEESLHHH HE

+
Macromolecule #38: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 9.355945 KDa
SequenceString:
MGAGTPSFGP RHNKTHTICR RCGNRCFHIQ KSKCAKCGYP DATMRHYDWE KKAHRKRAQG SGEMRHLKVT FAKLQRAYAP K

+
Macromolecule #39: Ribosomal protein L38e, putative

MacromoleculeName: Ribosomal protein L38e, putative / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 8.46896 KDa
SequenceString:
MPKQVTTPKE FLALAARSDA KWVKVKKASD DNTKFKLRTT KYLYTLSVKP KFVELVQNSI PESLEVIVLD KKAE

+
Macromolecule #40: 60S ribosomal protein L39, putative

MacromoleculeName: 60S ribosomal protein L39, putative / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 6.297775 KDa
SequenceString:
MAAHKTLRTK RILCKAMKVN RPMPQFIRQM TGVRHIRNPL TRHWRRRKMN I

+
Macromolecule #41: Ubiquitin, putative

MacromoleculeName: Ubiquitin, putative / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 15.101515 KDa
SequenceString:
MQIFVKTLTG KHITLEVEPT DRIEDVKAKI QDKEGIPPDQ QRLIFAGKQL EDGNTLQDYS IQKDSTLHLV LRLRGGGKEA YEPSLRILA EKTNVKRSVC RKCYARLSER AQQCRHKQCG HCKSIRPKKA GKE

+
Macromolecule #42: 60S ribosomal protein L44, putative

MacromoleculeName: 60S ribosomal protein L44, putative / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 12.471015 KDa
SequenceString:
MVHHPKTKKF FCKFCKKHTM CKVSQASRGE ERHSALGRRR YDRKQKGFGG RVKPILRRSK KTSKKISLRF TCQSCKKATM YCLGRLRKF EWTVEKKKKH AEPTW

+
Macromolecule #43: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichomonas vaginalis (eukaryote)
Molecular weightTheoretical: 10.051994 KDa
SequenceString:
MARRTKKVGI CGKYGVRYGA SLRKTIKKIE ISQRKKYPCA FCGKDSVKRN AVGIWTCTKC GKAVAGGAYT PTTPAALTAR AAYKRIREA QAK

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 2.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 57162
FSC plot (resolution estimation)

+
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